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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ADH1B Monoclonal antibody (66939-1-Ig)

    WB analysis using 66939-1-Ig (same clone as 66939-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66939-1-Ig (ADH1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66939-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using ADH1B Monoclonal antibody (66939-1-Ig)

    WB analysis of HepG2 using 66939-1-Ig (same clone as 66939-1-PBS)

    WB result of ADH1B antibody (66939-1-Ig; 1:50000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ADH1B transfected HepG2 cells. This data was developed using the same antibody clone with 66939-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig liver tissue using ADH1B Monoclonal antibody (66939-1-Ig)

    WB analysis of pig liver using 66939-1-Ig (same clone as 66939-1-PBS)

    pig liver tissue were subjected to SDS PAGE followed by western blot with 66939-1-Ig (ADH1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66939-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat liver tissue using ADH1B Monoclonal antibody (66939-1-Ig)

    WB analysis of rat liver using 66939-1-Ig (same clone as 66939-1-PBS)

    rat liver tissue were subjected to SDS PAGE followed by western blot with 66939-1-Ig (ADH1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66939-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ADH1B Monoclonal antibody (66939-1-Ig)

    IHC staining of human liver cancer using 66939-1-Ig (same clone as 66939-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66939-1-Ig (ADH1B antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66939-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ADH1B Monoclonal antibody (66939-1-Ig)

    IHC staining of human liver cancer using 66939-1-Ig (same clone as 66939-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66939-1-Ig (ADH1B antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66939-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ADH1B Monoclonal antibody (66939-1-Ig)

    IF Staining of HepG2 using 66939-1-Ig (same clone as 66939-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ADH1B antibody (66939-1-Ig, Clone: 1F1B4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66939-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

ADH1B Monoclonal antibody, PBS Only

ADH1B Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66939-1-PBS
Clone No.1F1B4

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

ADH1B, Alcohol dehydrogenase 1B, DKFZp686C06125

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66939-1-PBS targets ADH1B in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity Human, Mouse, Rat, Pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen ADH1B fusion protein Ag10630 相同性解析による交差性が予測される生物種
Full Name alcohol dehydrogenase 1B (class I), beta polypeptide
Calculated molecular weight 375 aa, 40 kDa
Observed molecular weight 35-40 kDa
GenBank accession numberBC033009
Gene Symbol ADH1B
Gene ID (NCBI) 125
RRIDAB_2882263
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP00325
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

The ADH1B gene encodes the beta subunit of class I alcohol dehydrogenase (ADH), an enzyme that catalyzes the rate-limiting step for ethanol metabolism: the oxidation of alcohol to acetaldehyde. Class 1 ADH is a homo- or heterodimeric molecule, formed by the association of 3 types of class I ADH subunits, alpha (ADH1A), beta, and gamma(ADH1C). ADH1B is also named as ADH2 and belongs to the zinc-containing alcohol dehydrogenase family. This protein can exsit as a homodimer(PMID:19365573).