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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ADH1C Polyclonal antibody

ADH1C Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 30621-1-AP

Synonyms

ADH1C, Alcohol dehydrogenase 1C

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ADH1C Polyclonal antibody (30621-1-AP)

    WB analysis using 30621-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30621-1-AP (ADH1C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inmouse testis tissue, mouse colon tissue, rat colon tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30621-1-AP targets ADH1C in WB, ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ADH1C fusion protein Ag33037 相同性解析による交差性が予測される生物種
Full Name alcohol dehydrogenase 1C (class I), gamma polypeptide
Calculated molecular weight 375 aa, 40 kDa
Observed molecular weight40 kDa
GenBank accession numberBC062476
Gene symbol ADH1C
Gene ID (NCBI) 126
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Alcohol dehydrogenase 1C(ADH1C), also known as ADH3, is an alcohol dehydrogenase that can catalyze ethanol oxidation to metabolite acetaldehyde. According to THE HUMAN PROTEIN ATLAS database, ADH1C locates mainly to the cytosol and plasma membrane. Genetic polymorphism of the ADH1C gene (ADH1C*1 allele) is associated with various human cancers, such as gastric cancer, oral cancer and CRC, for it encodes an alcohol dehydrogenase producing 2.5 times more acetaldehyde.(PMID: 35300114)

Protocols

Product Specific Protocols
WB protocol for ADH1C antibody 30621-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols