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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ADHFE1 Polyclonal antibody

ADHFE1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14674-1-AP

Synonyms

HOT, HMFT2263, Fe-containing alcohol dehydrogenase, EC:1.1.99.24, Alcohol dehydrogenase iron-containing protein 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ADHFE1 Polyclonal antibody (14674-1-AP)

    WB analysis using 14674-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14674-1-AP (ADHFE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using ADHFE1 Polyclonal antibody (14674-1-AP)

    IHC staining of human colon using 14674-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14674-1-AP (ADHFE1 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using ADHFE1 Polyclonal antibody (14674-1-AP)

    IHC staining of human colon using 14674-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14674-1-AP (ADHFE1 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using ADHFE1 Polyclonal antibody (14674-1-AP)

    IHC staining of human liver using 14674-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14674-1-AP (ADHFE1 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using ADHFE1 Polyclonal antibody (14674-1-AP)

    IHC staining of human liver using 14674-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14674-1-AP (ADHFE1 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ADHFE1 Polyclonal antibody (14674-1-AP)

    IHC staining of human colon cancer using 14674-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14674-1-AP (ADHFE1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ADHFE1 Polyclonal antibody (14674-1-AP)

    IHC staining of human colon cancer using 14674-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14674-1-AP (ADHFE1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHT-29 cells, mouse kidney tissue, mouse liver tissue, rat kidney tissue
Positive IHC detected inhuman colon tissue, human colon cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14674-1-AP targets ADHFE1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ADHFE1 fusion protein Ag6382 相同性解析による交差性が予測される生物種
Full Name alcohol dehydrogenase, iron containing, 1
Calculated molecular weight 50 kDa
Observed molecular weight 50 kDa, 40-45 kDa
GenBank accession numberBC064634
Gene symbol ADHFE1
Gene ID (NCBI) 137872
RRIDAB_2221451
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The ADHFE1 gene encodes hydroxyacid-oxoacid transhydrogenase which is responsible for the oxidation of 4-hydroxybutyrate in mammalian tissues. It belongs to the iron-containing alcohol dehydrogenase family. ADHFE1 has 4 isoforms produced by alternative splicing with the MW of 50, 45, 32, 27 kDa.

Protocols

Product Specific Protocols
WB protocol for ADHFE1 antibody 14674-1-APDownload protocol
IHC protocol for ADHFE1 antibody 14674-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Circ Cardiovasc Genet

Proteome-Based Systems Biology Analysis of the Diabetic Mouse Aorta Reveals Major Changes in Fatty Acid Biosynthesis as Potential Hallmark in Diabetes Associated Vascular Disease.

Authors - Holger Husi
View Article