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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ADHFE1 Polyclonal antibody

ADHFE1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19359-1-AP

Synonyms

ADH8, ADHFE1, HMFT2263, HOT

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse heart tissue using ADHFE1 Polyclonal antibody (19359-1-AP)

    WB analysis of mouse heart using 19359-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 19359-1-AP (ADHFE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using ADHFE1 Polyclonal antibody (19359-1-AP)

    WB analysis of mouse kidney using 19359-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 19359-1-AP (ADHFE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using ADHFE1 Polyclonal antibody (19359-1-AP)

    WB analysis of mouse heart using 19359-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 19359-1-AP (ADHFE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat kidney tissue using ADHFE1 Polyclonal antibody (19359-1-AP)

    WB analysis of rat kidney using 19359-1-AP

    rat kidney tissue were subjected to SDS PAGE followed by western blot with 19359-1-AP (ADHFE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using ADHFE1 Polyclonal antibody (19359-1-AP)

    WB analysis of mouse kidney using 19359-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 19359-1-AP (ADHFE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse heart tissue using ADHFE1 Polyclonal antibody (19359-1-AP)

    IP experiment of mouse heart using 19359-1-AP

    IP result of anti-ADHFE1 (IP:19359-1-AP, 4ug; Detection:19359-1-AP 1:300) with mouse heart tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human colon tissue using ADHFE1 Polyclonal antibody (19359-1-AP)

    IHC staining of human colon using 19359-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 19359-1-AP (ADHFE1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using ADHFE1 Polyclonal antibody (19359-1-AP)

    IHC staining of human colon using 19359-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 19359-1-AP (ADHFE1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ADHFE1 Polyclonal antibody (19359-1-AP)

    IHC staining of human colon cancer using 19359-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 19359-1-AP (ADHFE1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ADHFE1 Polyclonal antibody (19359-1-AP)

    IHC staining of human colon cancer using 19359-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 19359-1-AP (ADHFE1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using ADHFE1 Polyclonal antibody (19359-1-AP)

    IHC staining of human liver using 19359-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19359-1-AP (ADHFE1 Antibody) at dilution of 1:50(under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using ADHFE1 Polyclonal antibody (19359-1-AP)

    IHC staining of human liver using 19359-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19359-1-AP (ADHFE1 Antibody) at dilution of 1:50(under 40x lens).

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Tested Applications

Positive WB detected inmouse heart tissue, mouse kidney tissue, rat kidney tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman colon tissue, human colon cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 19359-1-AP is ADHFE1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ADHFE1 fusion protein Ag6803 相同性解析による交差性が予測される生物種
Full Name alcohol dehydrogenase, iron containing, 1
Calculated molecular weight 50 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC064634
Gene symbol ADHFE1
Gene ID (NCBI) 137872
RRIDAB_10644280
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ADHFE1 antibody 19359-1-APDownload protocol
IHC protocol for ADHFE1 antibody 19359-1-APDownload protocol
IP protocol for ADHFE1 antibody 19359-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols