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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Adiponectin Recombinant antibody (83961-4-RR)

    WB analysis using 83961-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83961-4-RR (Adiponectin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83961-4-RR

    Affinity and Kinetic Characterization of 83961-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83961-4-RR against Human ADIPOQ were performed. The affinity constant is below 1 pM.

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Adiponectin Recombinant antibody

Adiponectin Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 83961-4-RR
Clone No.241082C6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

ADIPOQ, ADIPQTL1, Adipocyte, C1q and collagen domain-containing protein, Adipocyte complement-related 30 kDa protein, 30 kDa adipocyte complement-related protein

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inhuman adipose tissue, human plasma

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83961-4-RR targets Adiponectin in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Adiponectin fusion protein Ag16304 相同性解析による交差性が予測される生物種
Full Name adiponectin, C1Q and collagen domain containing
Calculated molecular weight 244 aa, 26 kDa
Observed molecular weight29 kDa
GenBank accession numberBC096308
Gene Symbol Adiponectin
Gene ID (NCBI) 9370
RRIDAB_3671542
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ15848
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Adiponectin (AdipoQ), an adipocyte-derived hormone, is one of the most abundant adipokines in the blood circulation. Adiponectin modulates a number of metabolic processes, including improving INS sensitivity and anti-inflammatory activity. The role of AdipoQ in reproduction is not yet fully understood, but the expression of AdipoQ in reproductive tissues has been observed in various animals and humans, including chicken testis, bovine ovary, and human placenta. Adiponectin exerts its effects by activating a range of different signaling molecules via binding to two transmembrane AdipoQ receptors, AdipoR1 and AdipoR2. AdipoR1 is expressed primarily in the skeletal muscle, whereas AdipoR2 is predominantly expressed in the liver. AdipoQ May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors.

Protocols

Product Specific Protocols
WB protocol for Adiponectin antibody 83961-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols