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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ADK Monoclonal antibody (66929-1-Ig)

    WB analysis using 66929-1-Ig (same clone as 66929-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66929-1-Ig (ADK antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66929-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ADK Monoclonal antibody (66929-1-Ig)

    IHC staining of human liver cancer using 66929-1-Ig (same clone as 66929-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66929-1-Ig (ADK antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66929-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ADK Monoclonal antibody (66929-1-Ig)

    IHC staining of human liver cancer using 66929-1-Ig (same clone as 66929-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66929-1-Ig (ADK antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66929-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using ADK Monoclonal antibody (66929-1-Ig)

    IF Staining of human liver cancer using 66929-1-Ig (same clone as 66929-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using ADK antibody (66929-1-Ig, Clone: 1G1A5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66929-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using ADK Monoclonal antibody (66929-1-Ig)

    IF Staining of human breast cancer using 66929-1-Ig (same clone as 66929-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using ADK antibody (66929-1-Ig, Clone: 1G1A5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66929-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of NIH/3T3 cells using ADK Monoclonal antibody (66929-1-Ig)

    FC experiment of NIH/3T3 using 66929-1-Ig (same clone as 66929-1-PBS)

    1X10^6 NIH/3T3 cells were intracellularly stained with 0.4 ug Anti-Human ADK (66929-1-Ig, Clone:1G1A5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66929-1-PBS in a different storage buffer formulation.

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ADK Monoclonal antibody, PBS Only

ADK Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), Indirect ELISA
Cat No. 66929-1-PBS
Clone No.1G1A5

Host / Isotype

Mouse / IgG1

Reactivity

rat, mouse, human

Applications

WB, IHC, IF-P, FC (Intra), Indirect ELISA

Synonyms

EC:2.7.1.20, AK, adenosine kinase, Adenosine 5'-phosphotransferase, 1G1A5

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66929-1-PBS targets ADK in WB, IHC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with rat, mouse, human samples.

Tested Reactivity rat, mouse, human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ADK fusion protein Ag7466 相同性解析による交差性が予測される生物種
Full Name adenosine kinase
Calculated molecular weight 41 kDa
Observed molecular weight 40-46 kDa
GenBank accession numberBC003568
Gene Symbol ADK
Gene ID (NCBI) 132
RRIDAB_2882255
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP55263
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.