☆ 3/31(月)まで!二次抗体 & FlexAbleが30%オフ! >> 免疫アッセイサポートキャンペーン

Adrenomedullin Polyclonal antibody

Adrenomedullin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 10778-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

ADM, ProAM N terminal 20 peptide, Proadrenomedullin N-20 terminal peptide, Pro-adrenomedullin, PAMP

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration:  150 μL, 600 ug/ml
20 μL, 600 ug/ml
150 μL, 600 ug/ml

¥67,000/ 150 μL

本製品の国内在庫をすぐに見る(表示に数秒かかります)

【全製品対象☆】小さなギフトがついてくる!春の特典キャンペーン開催中

他社製品の代替品をお探しですか?『抗体代替品/同等品検索システム』でお調べいただけます


ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。


国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inhuman kidney tissue, fetal human brain tissue, Raji cells, A549 cells, human placenta tissue
Positive IHC detected inhuman placenta tissue, human pancreas cancer tissue, human kidney tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10778-1-AP targets Adrenomedullin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Adrenomedullin fusion protein Ag1197 相同性解析による交差性が予測される生物種
Full Name adrenomedullin
Calculated molecular weight 20 kDa
Observed molecular weight 6 kDa
GenBank accession numberBC015961
Gene Symbol Adrenomedullin/ADM
Gene ID (NCBI) 133
RRIDAB_2242255
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP35318
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Adrenomedullin (AM) and proadrenomedullin N-terminal 20 peptide (PAMP) are two small active hormones derived from the expression of a single gene (Adm) that is expressed throughout the GI tract, including the mucosal epithelium, glandular duct cells, neuroendocrine cells, and smooth muscle cells of the GI tract, between the oral cavity and the rectum (PMID:10782362, PMID:27345325). These two peptides coexist in GI cells, where they regulate many physiological functions including vasodilation, angiogenesis, anti-inflammation, organ protection, and tissue repair. AM suppresses inflammatory cytokine production in the intestinal mucosa, improves vascular and lymphatic function, mucosal epithelial repair, and intestinal barrier function in animal models with intestinal inflammation (PMID:27965594, PMID:29311984). Molecular mass species of 18, 14, and 6 kDa were identified in tumor cell lysates and presumably represent AM precursor, processed intermediates, and the authentic peptide, respectively. There is also a 22-kDa immunoreactive species in two cancer cell lines, H720 and MCF-7 (PMID: 8798536).

Protocols

Product Specific Protocols
WB protocol for Adrenomedullin antibody 10778-1-APDownload protocol
IHC protocol for Adrenomedullin antibody 10778-1-APDownload protocol
IF protocol for Adrenomedullin antibody 10778-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Cells

Differentiation Trajectory of Limbal Stem and Progenitor Cells under Normal Homeostasis and upon Corneal Wounding.

Authors - Zhenwei Song
humanIHC

Cell Mol Life Sci

APE1 controls DICER1 expression in NSCLC through miR-33a and miR-130b.

Authors - Giulia Antoniali
humanWB

Prostate

Prostate fibroblasts enhance androgen receptor splice variant 7 expression in prostate cancer cells

Authors - Takeshi Sasaki
humanWB

CNS Neurosci Ther

Identification and validation of a glycolysis-related taxonomy for improving outcomes in glioma

Authors - Tianshu Ying
Loading...