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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ADPRH Polyclonal antibody

ADPRH Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17911-1-AP

Synonyms

ADP ribosylarginine hydrolase, ADPRH, ARH1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ADPRH Polyclonal antibody (17911-1-AP)

    WB analysis using 17911-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 17911-1-AP (ADPRH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using ADPRH Polyclonal antibody (17911-1-AP)

    WB analysis of mouse brain using 17911-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 17911-1-AP (ADPRH Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using ADPRH Polyclonal antibody (17911-1-AP)

    WB analysis of rat brain using 17911-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 17911-1-AP (ADPRH Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using ADPRH Polyclonal antibody (17911-1-AP)

    WB analysis of mouse brain using 17911-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 17911-1-AP (ADPRH Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using ADPRH Polyclonal antibody (17911-1-AP)

    IHC staining of mouse brain using 17911-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17911-1-AP (ADPRH antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using ADPRH Polyclonal antibody (17911-1-AP)

    IHC staining of mouse brain using 17911-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17911-1-AP (ADPRH antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

The immunogen of 17911-1-AP is ADPRH Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ADPRH fusion protein Ag12311 相同性解析による交差性が予測される生物種
Full Name ADP-ribosylarginine hydrolase
Calculated molecular weight 357 aa, 40 kDa
Observed molecular weight 36-39 kDa
GenBank accession numberBC063883
Gene symbol ADPRH
Gene ID (NCBI) 141
RRIDAB_2878466
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ADPRH antibody 17911-1-APDownload protocol
IHC protocol for ADPRH antibody 17911-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

J Cancer

ADPRH is a prognosis-related biomarker and correlates with immune infiltrates in low grade glioma.

Authors - Chunyu Zhang
View Article