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ADRA1A-Specific Polyclonal antibody
ADRA1A-Specific Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 19777-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse heart tissue, HeLa cells, mouse brain tissue |
Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 4 publications below |
IHC | See 1 publications below |
IF | See 1 publications below |
Product Information
19777-1-AP targets ADRA1A-Specific in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
Full Name | adrenergic, alpha-1A-, receptor |
Calculated molecular weight | 51 kDa |
Observed molecular weight | 52 kDa |
GenBank accession number | NM_000680 |
Gene symbol | ADRA1A |
Gene ID (NCBI) | 148 |
RRID | AB_10643238 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ADRA1A, also named as ADRA1C, belongs to the G-protein coupled receptor 1 family. ADRA1A mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Its effect is mediated by G(q) and G(11) proteins. The antibody is specific to ADRA1A.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for ADRA1A-Specific antibody 19777-1-AP | Download protocol |
FC protocol for ADRA1A-Specific antibody 19777-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Eur J Immunol Chronic stimulation desensitizes β2-adrenergic receptor responses in natural killer cells | ||
iScience Changes in transcriptomic landscape in human end-stage heart failure with distinct etiology. | ||
Pharmaceuticals (Basel) Vascular α1A Adrenergic Receptors as a Potential Therapeutic Target for IPAD in Alzheimer's Disease. | ||
Vascul Pharmacol The ERK1/2 pathway participates in the upregulation of the expression of mesenteric artery α1 receptors by intravenous tail injections of mmLDL in mice. | ||
Heliyon Luteolin improves vasoconstriction function and survival of septic mice via AMPK/NF-κB pathway | ||
BMC Neurosci Norepinephrine protects against cochlear outer hair cell damage and noise-induced hearing loss via α2A-adrenergic receptor |