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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ADRB1 Polyclonal antibody (28323-1-AP)

    WB analysis using 28323-1-AP (same clone as 28323-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28323-1-AP (ADRB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28323-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using ADRB1 Polyclonal antibody (28323-1-AP)

    IHC staining of mouse lung using 28323-1-AP (same clone as 28323-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 28323-1-AP (ADRB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28323-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

28323-1-PBS targets ADRB1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ADRB1 fusion protein Ag28223 相同性解析による交差性が予測される生物種
Full Name adrenergic, beta-1-, receptor
Calculated molecular weight 51 kDa
Observed molecular weight49 kDa
GenBank accession numberNM_000684
Gene Symbol ADRB1
Gene ID (NCBI) 153
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP08588
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ADRB1 (adrenergic receptor beta 1), a member of the G protein-coupled receptor (GPCR) superfamily, responds to catecholamine stimulation. ADRB1 is the predominant subtype expressed in the heart and mediates increases in inotropy and chronotropy when stimulated. ADRB1 mediates a wide range of cardiovascular physiological responses and has been of great interest to researchers as a therapeutic target for cardiovascular diseases. Moreover, three subtypes of ADRBs (ADRB1, ADRB2, and ADRB3) are encoded by three separate genes (PMID:16636683; 33372534; 33593484).