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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ADRM1 Monoclonal antibody (66464-1-Ig)

    WB analysis using 66464-1-Ig (same clone as 66464-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66464-1-Ig (ADRM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66464-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ADRM1 Monoclonal antibody (66464-1-Ig)

    IF Staining of HeLa using 66464-1-Ig (same clone as 66464-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ADRM1 antibody (66464-1-Ig, Clone: 1A1A3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66464-1-PBS in a different storage buffer formulation.

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ADRM1 Monoclonal antibody, PBS Only

ADRM1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 66464-1-PBS
Clone No.1A1A3

Host / Isotype

Mouse / IgG3

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

adhesion regulating molecule 1, ADRM1, ARM 1, ARM1, GP110, hRpn13, Rpn13, Rpn13 homolog

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66464-1-PBS targets ADRM1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Immunogen ADRM1 fusion protein Ag24699 相同性解析による交差性が予測される生物種
Full Name adhesion regulating molecule 1
Calculated molecular weight 407 aa, 42 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC017245
Gene Symbol ADRM1
Gene ID (NCBI) 11047
RRIDAB_2881832
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ16186
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

The 26S proteasome is a key component of the ubiquitin-proteasome system, a process responsible for the majority of cellular protein degradation. hRpn13 (also termed ADRM1 or GP110) is a novel 46-kDa subunit of its 19S regulatory complex. hRpn13 binds directly to the proteasome-associated deubiquitinating enzyme, UCH37, and enhances its isopeptidase activity. Overexpression of hRpn13 promotes the activity of the ubiquitin-proteasome system and modulates the influence of osteoblasts on osteoclasts by controlling the stability of regulatory proteins in osteoblasts.