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Species Reactivity

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Conjugate

ADRP/Perilipin-2 Monoclonal antibody, PBS Only

ADRP/Perilipin-2 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G5D4

Cat no : 60340-1-PBS

Synonyms

ADFP, Adipophilin, ADRP, ADRP/Perilipin 2, Perilipin 2, Perilipin-2, PLIN2

Validation Data Gallery

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  • Western Blot (WB) analysis of human adipose tissue using ADRP/Perilipin-2 Monoclonal antibody (60340-1-Ig)

    WB analysis of human adipose using 60340-1-Ig (same clone as 60340-1-PBS)

    human adipose tissue were subjected to SDS PAGE followed by western blot with 60340-1-Ig (ADRP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60340-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using ADRP/Perilipin-2 Monoclonal antibody (60340-1-Ig)

    IHC staining of human renal cell carcinoma using 60340-1-Ig (same clone as 60340-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 60340-1-Ig (ADRP Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60340-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using ADRP/Perilipin-2 Monoclonal antibody (60340-1-Ig)

    IHC staining of human renal cell carcinoma using 60340-1-Ig (same clone as 60340-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 60340-1-Ig (ADRP Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60340-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using ADRP/Perilipin-2 Monoclonal antibody (60340-1-Ig)

    IHC staining of human renal cell carcinoma using 60340-1-Ig (same clone as 60340-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 60340-1-Ig (ADRP/Perilipin 2 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60340-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using ADRP/Perilipin-2 Monoclonal antibody (60340-1-Ig)

    IHC staining of human renal cell carcinoma using 60340-1-Ig (same clone as 60340-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 60340-1-Ig (ADRP/Perilipin 2 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60340-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brown adipose tissue using ADRP/Perilipin-2 Monoclonal antibody (60340-1-Ig)

    IHC staining of mouse brown adipose using 60340-1-Ig (same clone as 60340-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 60340-1-Ig (ADRP/Perilipin 2 antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60340-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brown adipose tissue using ADRP/Perilipin-2 Monoclonal antibody (60340-1-Ig)

    IHC staining of mouse brown adipose using 60340-1-Ig (same clone as 60340-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 60340-1-Ig (ADRP/Perilipin 2 antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60340-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brown adipose tissue using ADRP/Perilipin-2 Monoclonal antibody (60340-1-Ig)

    IHC staining of rat brown adipose using 60340-1-Ig (same clone as 60340-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brown adipose slide using 60340-1-Ig (ADRP/Perilipin 2 antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60340-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60340-1-PBS targets ADRP/Perilipin-2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ADRP/Perilipin-2 fusion protein Ag7643 相同性解析による交差性が予測される生物種
Full Name adipose differentiation-related protein
Calculated molecular weight 48 kDa
Observed molecular weight 47-52 kDa
GenBank accession numberBC005127
Gene symbol ADFP
Gene ID (NCBI) 123
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ADRP (adipocyte differentiation related protein) also known as ADFP, adipophilin, or perilipin-2, is a member of PAT family which is responsible for the transportation of lipids and the formation of lipid droplets. ADRP is localized on the surface of lipid droplets in a variety of tissues and cell lines. ADRP is not detected in undifferentiated cells but increases rapidly to high levels when adipocyte precursors differentiate into adipocytes. Anti-ADRP antibody is a reliable and sensitive marker for lipid droplet. Enhanced expression of ADRP is linked to diseases with abnormal lipid storage, including hepatic steatosis, atherosclerosis and diabetes. Immunohistochemistry of ADRP may facilitate histomorphological diagnosis of these diseases.