AEBP2 Monoclonal antibody
AEBP2 Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, pig
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
7H2B11
Cat no : 66141-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
The immunogen of 66141-1-Ig is AEBP2 Fusion Protein expressed in E. coli.
Tested Reactivity | human, pig |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | AEBP2 fusion protein Ag17905 相同性解析による交差性が予測される生物種 |
Full Name | AE binding protein 2 |
Calculated molecular weight | 33 kDa, 54 kDa |
Observed molecular weight | 55 kDa |
GenBank accession number | BC015624 |
Gene symbol | AEBP2 |
Gene ID (NCBI) | 121536 |
RRID | AB_2881538 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
AEBP2 belongs to the AEBP2/jing C2H2-type zinc-finger family. It is a DNA-binding transcriptional repressor. AEBP2 is a zinc finger protein that has been shown to interact with the mammalian Polycomb Repression Complex 2 (PRC2). It may interact with and stimulate the activity of the PRC2 complex, which methylates 'Lys-9' and 'Lys-27' residues of histone H3. AEBP2 is an evolutionarily well-conserved gene that is found in the animals ranging from flying insects to mammals. (PMID:19293275) The antibody recognizes adult-specific larger form (52 kDa) and the embryo-specific smaller form (31 kDa).
Protocols
Product Specific Protocols | |
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WB protocol for AEBP2 antibody 66141-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |