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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AES Polyclonal antibody (23913-1-AP)

    WB analysis using 23913-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23913-1-AP (AES antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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AES Polyclonal antibody

AES Polyclonal Antibody for WB, ELISA
Cat No. 23913-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

AES, AES 1, AES 2, Amino enhancer of split, ESP1, Gp130 associated protein GAM, GRG, GRG5, Protein ESP1, Protein GRG, TLE5

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inDU 145 cells, mouse skeletal muscle tissue, rat skeletal muscle tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23913-1-AP targets AES in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AES fusion protein Ag21021 相同性解析による交差性が予測される生物種
Full Name amino-terminal enhancer of split
Calculated molecular weight 264 aa, 29 kDa
Observed molecular weight 22-29 kDa
GenBank accession numberBC113735
Gene Symbol AES
Gene ID (NCBI) 166
RRIDAB_3669425
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ08117
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Amino-terminal enhancer of split (AES), belongs to the groucho/TLE family of proteins, can function as a homooligomer or as a heteroologimer with other family members in order to forcefully repress the expression of other family member genes. AES can repress NFkB-regulated gene expression and is believed to have a significant role in initiating and maintaining cell differentiation events. AES has been found to suppress local invasion and intravasation through inhibition of the Notch pathway, leading to preventing the metastatic spread of endogenous tumors(PMID: 33754069).

Protocols

Product Specific Protocols
WB protocol for AES antibody 23913-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols