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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AES Recombinant antibody (83878-5-RR)

    WB analysis using 83878-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83878-5-RR (AES antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83878-5-RR

    Affinity and Kinetic Characterization of 83878-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83878-5-RR against Human AES were performed. The affinity constant is 1.76 nM.

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AES Recombinant antibody

AES Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 83878-5-RR
Clone No.240987D9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

TLE5, Gp130-associated protein GAM, ESP1, Amino-terminal enhancer of split, AES 2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, K-562 cells, DU 145 cells, MDA-MB-231 cells, NIH/3T3 cells, mouse skeletal muscle tissue, rat skeletal muscle tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83878-5-RR targets AES in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen AES fusion protein Ag21021 相同性解析による交差性が予測される生物種
Full Name amino-terminal enhancer of split
Calculated molecular weight 264 aa, 29 kDa
Observed molecular weight 22-29 kDa
GenBank accession numberBC113735
Gene Symbol AES
Gene ID (NCBI) 166
RRIDAB_3671462
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ08117
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Amino-terminal enhancer of split (AES), belongs to the groucho/TLE family of proteins, can function as a homooligomer or as a heteroologimer with other family members in order to forcefully repress the expression of other family member genes. AES can repress NFkB-regulated gene expression and is believed to have a significant role in initiating and maintaining cell differentiation events. AES has been found to suppress local invasion and intravasation through inhibition of the Notch pathway, leading to preventing the metastatic spread of endogenous tumors(PMID: 33754069).

Protocols

Product Specific Protocols
WB protocol for AES antibody 83878-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols