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  • KD/KO Validated

AFAP1L2 Polyclonal antibody

AFAP1L2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Cat no : 17183-1-AP

Synonyms

AFAP1 like protein 2, AFAP1L2, KIAA1914, XB130


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Tested Applications

Positive WB detected inA549 cells, mouse thymus tissue
Positive IP detected inmouse thymus tissue
Positive IHC detected inhuman thymus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17183-1-AP targets AFAP1L2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AFAP1L2 fusion protein Ag10966 相同性解析による交差性が予測される生物種
Full Name actin filament associated protein 1-like 2
Calculated molecular weight 818 aa, 91 kDa
Observed molecular weight 130 kDa
GenBank accession numberBC033212
Gene symbol AFAP1L2
Gene ID (NCBI) 84632
RRIDAB_2226101
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AFAP1L2, also named as Actin filament-associated protein 1-like 2 or XB130, is a 818 amino acid protein, which contains two PH domains. AFAP1L2 is detected in spleen and thyroid, and at lower levels in kidney, brain, lung and pancreas. AFAP1L2 localizes in the cytoplasm and may play a role in a signaling cascade by enhancing the kinase activity of SRC. AFAP1L2 promotes thyroid tumor growth, and its expression in these cancer cells may affect cell proliferation and survival by controlling the expression of multiple genes, especially transcription regulators.

Protocols

Product Specific Protocols
WB protocol for AFAP1L2 antibody 17183-1-APDownload protocol
IHC protocol for AFAP1L2 antibody 17183-1-APDownload protocol
IP protocol for AFAP1L2 antibody 17183-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Transl Oncol

Identification of genes associated with local aggressiveness and metastatic behavior in soft tissue tumors.

Authors - Cunha Isabela Werneck IW
mouseIHC

Carcinogenesis

XB130 deficiency enhances carcinogen-induced skin tumorigenesis.

Authors - Hae-Ra Cho
  • KO Validated
mouseWB

Mol Cell

PLEKHS1 drives PI3Ks and remodels pathway homeostasis in PTEN-null prostate

Authors - Tamara A M Chessa
humanWB

Oncol Lett

The 3'‑untranslated region of XB130 regulates its mRNA stability and translational efficiency in non‑small cell lung cancer cells

Authors - Qinrong Wang
mouseIF,WB,IHC

Cell Rep

Integrative multi-omic profiling of adult mouse brain endothelial cells and potential implications in Alzheimer's disease

Authors - Min Yu
humanWB,IF

Autophagy

Artesunate Sensitizes human hepatocellular carcinoma to sorafenib via exacerbating AFAP1L2-SRC-FUNDC1 axis-dependent mitophagy

Authors - Zhaochen Ma

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Tamara (Verified Customer) (12-02-2020)

antibody works very well in detecting endogenous Afap1l2 protein in mouse prostate lysates

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: mouse prostate