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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

AFF4 Polyclonal antibody

AFF4 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 13197-1-AP

Synonyms

AF4/FMR2 family member 4, AF4/FMR2 family, member 4, AF5Q31, AFF4, MCEF, Protein AF 5q31

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using AFF4 Polyclonal antibody (13197-1-AP)

    WB analysis of Jurkat using 13197-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 13197-1-AP (AFF4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using AFF4 Polyclonal antibody (13197-1-AP)

    WB analysis of HL-60 using 13197-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 13197-1-AP (AFF4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inJurkat cells, HL-60 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13197-1-AP targets AFF4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AFF4 fusion protein Ag3931 相同性解析による交差性が予測される生物種
Full Name AF4/FMR2 family, member 4
Calculated molecular weight 365 aa, 40 kDa
Observed molecular weight 150 kDa
GenBank accession numberBC025700
Gene symbol AFF4
Gene ID (NCBI) 27125
RRIDAB_2226131
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for AFF4 antibody 13197-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols