• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using AFG3L2 Polyclonal antibody (14631-1-AP)

    WB analysis using 14631-1-AP (same clone as 14631-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14631-1-AP (AFG3L2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14631-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using AFG3L2 Polyclonal antibody (14631-1-AP)

    WB analysis using 14631-1-AP (same clone as 14631-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14631-1-AP (AFG3L2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14631-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using AFG3L2 Polyclonal antibody (14631-1-AP)

    WB analysis of HeLa using 14631-1-AP (same clone as 14631-1-PBS)

    WB result of AFG3L2 antibody (14631-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AFG3L2 transfected HeLa cells. This data was developed using the same antibody clone with 14631-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse kidney tissue using AFG3L2 Polyclonal antibody (14631-1-AP)

    WB analysis of mouse kidney using 14631-1-AP (same clone as 14631-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14631-1-AP (AFG3L2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14631-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using AFG3L2 Polyclonal antibody (14631-1-AP)

    WB analysis of mouse skeletal muscle using 14631-1-AP (same clone as 14631-1-PBS)

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14631-1-AP (AFG3L2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14631-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using AFG3L2 Polyclonal antibody (14631-1-AP)

    IP experiment of HeLa using 14631-1-AP (same clone as 14631-1-PBS)

    IP result of anti-AFG3L2 (IP:14631-1-AP, 4ug; Detection:14631-1-AP 1:5000) with HeLa cells lysate 1560 ug. This data was developed using the same antibody clone with 14631-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using AFG3L2 Polyclonal antibody (14631-1-AP)

    IHC staining of human kidney using 14631-1-AP (same clone as 14631-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14631-1-AP (AFG3L2 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 14631-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using AFG3L2 Polyclonal antibody (14631-1-AP)

    IHC staining of human kidney using 14631-1-AP (same clone as 14631-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14631-1-AP (AFG3L2 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 14631-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using AFG3L2 Polyclonal antibody (14631-1-AP)

    IHC staining of human kidney using 14631-1-AP (same clone as 14631-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 14631-1-AP (AFG3L2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14631-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using AFG3L2 Polyclonal antibody (14631-1-AP)

    IHC staining of human kidney using 14631-1-AP (same clone as 14631-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 14631-1-AP (AFG3L2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14631-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using AFG3L2 Polyclonal antibody (14631-1-AP)

    IF Staining of HepG2 using 14631-1-AP (same clone as 14631-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using AFG3L2 antibody 14631-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 14631-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using AFG3L2 Polyclonal antibody (14631-1-AP)

    IF Staining of HeLa using 14631-1-AP (same clone as 14631-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 14631-1-AP (AFG3L2 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14631-1-PBS in a different storage buffer formulation.

☆ 3/31（月）まで！二次抗体 & FlexAbleが30%オフ！ >> 免疫アッセイサポートキャンペーン

  • Featured Product
  • KD/KO Validated

AFG3L2 Polyclonal antibody, PBS Only

AFG3L2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 14631-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14631-1-PBS targets AFG3L2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AFG3L2 fusion protein Ag6209 相同性解析による交差性が予測される生物種
Full Name AFG3 ATPase family gene 3-like 2 (yeast)
Calculated molecular weight 88 kDa
Observed molecular weight 80-90 kDa
GenBank accession numberBC065016
Gene Symbol AFG3L2
Gene ID (NCBI) 10939
RRIDAB_2242420
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y4W6
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

AFG3L2 is the catalytic subunit of the m-AAA protease, an ATP-dependent proteolytic complex of the mitochondrial inner membrane that degrades misfolded proteins and regulates ribosome assembly(PMID:17101804). Human AFG3L2 is an 80-kDa protein encoded by a 17-exon gene and highly and selectively expressed in human cerebellar Purkinje cells(PMID:20208537) and it can exsit as a truncated 65 kDa protein(PMID:18337413). Defects in AFG3L2 are the cause of spinocerebellar ataxia type 28 (SCA28) and spastic ataxia autosomal recessive type 5 (SPAX5).