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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

AFM Polyclonal antibody, PBS Only

AFM Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 19494-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using AFM Polyclonal antibody (19494-1-AP)

    WB analysis of human plasma using 19494-1-AP (same clone as 19494-1-PBS)

    human blood were subjected to SDS PAGE followed by western blot with 19494-1-AP (AFM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19494-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using AFM Polyclonal antibody (19494-1-AP)

    IF Staining of HeLa using 19494-1-AP (same clone as 19494-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using AFM antibody (19494-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 19494-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

19494-1-PBS targets AFM in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name afamin
Calculated molecular weight 69 kDa
Observed molecular weight 85 kDa
GenBank accession numberNM_001133
Gene symbol AFM
Gene ID (NCBI) 173
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

AFM (Afamin) is a member of the albumin superfamily, which comprises albumin, vitamin D-binding protein, a-fetoprotein, and afamin. AFM is present in the plasma/serum, cerebrospinal fluid, and follicular fluid, and functions as a vitamin E-binding protein. Alterations of expression level of AFM have been linked to variety of diseases like cancer.