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Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using AFM Monoclonal antibody (66113-1-Ig)

    WB analysis of human plasma using 66113-1-Ig (same clone as 66113-1-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 66113-1-Ig (AFM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66113-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of human plasma using AFM Monoclonal antibody (66113-1-Ig)

    IP experiment of human plasma using 66113-1-Ig (same clone as 66113-1-PBS)

    IP result of anti-AFM (IP:66113-1-Ig, 5ug; Detection:66113-1-Ig 1:500) with human plasma lysate 4000ug. This data was developed using the same antibody clone with 66113-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using AFM Monoclonal antibody (66113-1-Ig)

    IHC staining of mouse brain using 66113-1-Ig (same clone as 66113-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66113-1-Ig (AFM antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66113-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using AFM Monoclonal antibody (66113-1-Ig)

    IHC staining of mouse brain using 66113-1-Ig (same clone as 66113-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66113-1-Ig (AFM antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66113-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using AFM Monoclonal antibody (66113-1-Ig)

    IHC staining of human liver using 66113-1-Ig (same clone as 66113-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66113-1-Ig (AFM Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66113-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using AFM Monoclonal antibody (66113-1-Ig)

    IHC staining of human liver using 66113-1-Ig (same clone as 66113-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66113-1-Ig (AFM Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66113-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using AFM Monoclonal antibody (66113-1-Ig)

    IF Staining of HeLa using 66113-1-Ig (same clone as 66113-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using AFM antibody (66113-1-Ig, Clone: 2G9F6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66113-1-PBS in a different storage buffer formulation.

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AFM Monoclonal antibody, PBS Only

AFM Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 66113-1-PBS
Clone No.2G9F6

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

ALF, ALBA, ALB2, afamin/AFM, afamin

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66113-1-PBS targets AFM in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen AFM fusion protein Ag16866 相同性解析による交差性が予測される生物種
Full Name afamin
Calculated molecular weight 599 aa, 69 kDa
Observed molecular weight 85 kDa
GenBank accession numberBC109020
Gene Symbol AFM
Gene ID (NCBI) 173
RRIDAB_2881512
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP43652
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

AFM (Afamin) is a member of the albumin superfamily, which comprises albumin, vitamin D-binding protein, a-fetoprotein, and afamin. AFM is present in the plasma/serum, cerebrospinal fluid, and follicular fluid, and functions as a vitamin E-binding protein. Alterations of expression level of AFM have been linked to variety of diseases like cancer.