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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

AFP Polyclonal antibody

AFP Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 32214-1-AP

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AFP Polyclonal antibody (32214-1-AP)

    WB analysis using 32214-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32214-1-AP (AFP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using AFP Polyclonal antibody (32214-1-AP)

    IHC staining of human hepatocirrhosis using 32214-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 32214-1-AP (AFP antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using AFP Polyclonal antibody (32214-1-AP)

    IHC staining of human hepatocirrhosis using 32214-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 32214-1-AP (AFP antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHepG2 cells
Positive IHC detected inhuman hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32214-1-AP targets AFP in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name alpha-fetoprotein
Calculated molecular weight 69 kDa
Observed molecular weight68~72 kDa
GenBank accession numberBC027881
Gene symbol AFP
Gene ID (NCBI) 174
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AFP (Alpha-fetoprotein) is a major plasma protein in the fetus and its concentration is very low in the adult (PMID:24120489). AFP can be detected at abnormally high concentrations in hepatocellular carcinomas as well as in the plasma and ascitic fluid of adults with hepatoma, indicating that AFP can serve as a tumor marker (PMID: 18669658). AFP is also a glycosylated protein and based on its binding capability to lectin Lens Culinaris Agglutinin (LCA), and total AFP can be separated into three different glycoforms, AFP-L1, AFP-L2, and AFP-L3. Core-fucosylated form of AFP (AFP-L3) is a more specific indicator than total AFP for HCC (PMID: 33128033, 35458505)

Protocols

Product Specific Protocols
WB protocol for AFP antibody 32214-1-APDownload protocol
IHC protocol for AFP antibody 32214-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols