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Host
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Species Reactivity
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Species Reactivity
0
Conjugate
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Conjugate
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Compatible Species
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

AFP Recombinant antibody, PBS Only (Capture)

AFP Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

230435G1

Cat no : 82982-3-PBS

Synonyms

Alpha-fetoprotein, Alpha-fetoglobulin, Alpha-1-fetoprotein, alpha fetoprotein, Alpha fetoglobulin

Validation Data Gallery

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  • Sandwich ELISA standard curve of MP00001-4

    Sandwich ELISA standard curve of MP00001-4

    Sandwich ELISA standard curve of MP00001-4, AFP Recombinant Matched Antibody Pair - PBS only. 82982-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0539. 82982-4-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL.

  • Sample test of MP00001-4

    Sample test of MP00001-4

    HepG2 (human hepatocellular carcinoma cells) were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. The mean AFP concentration was determined to be 3,038.3 ng/mL in HepG2 supernatant.

  • Sample test of MP00001-4

    Sample test of MP00001-4

    Serum of sixteen individual healthy human donors was measured. The AFP concentration of detected samples was determined to be 8,740.7 pg/mL with a range of 2,872.1 - 32,900 pg/mL.

  • Cytometric bead array standard curve of MP00001-2

    Cytometric bead array standard curve of MP00001-2

    Cytometric bead array standard curve of MP00001-2, AFP Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82982-3-PBS. Detection antibody: 82982-2-PBS. Standard: Eg0539. Range: 1.25-20 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82982-3-PBS targets AFP as part of a matched antibody pair:

MP00001-2: 82982-3-PBS capture and 82982-2-PBS detection (validated in Cytometric bead array)

MP00001-4: 82982-3-PBS capture and 82982-4-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name alpha-fetoprotein
Calculated molecular weight 69 kDa
GenBank accession numberBC027881
Gene symbol AFP
Gene ID (NCBI) 174
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer 100% PBS pH 7.3
Storage ConditionsStore at -80°C.