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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

AFP Recombinant antibody

AFP Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

230435A2

Cat no : 82982-5-RR

Synonyms

Alpha-fetoprotein, Alpha-fetoglobulin, Alpha-1-fetoprotein, alpha fetoprotein, Alpha fetoglobulin

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using AFP Recombinant antibody (82982-5-RR)

    WB analysis of HepG2 using 82982-5-RR

    HepG2 cells were subjected to SDS PAGE followed by western blot with 82982-5-RR (AFP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using AFP Recombinant antibody (82982-5-RR)

    WB analysis using 82982-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82982-5-RR (AFP antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using AFP Recombinant antibody (82982-5-RR)

    IF Staining of HepG2 using 82982-5-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using AFP antibody (82982-5-RR, Clone: 230435A2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 82982-5-RR

    Affinity and Kinetic Characterization of 82982-5-RR

    Biolayer interferometry (BLl) kinetic assays of 82982-5-RR against Human AFP were performed. The affinity constant is 0.696 nM.

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Tested Applications

Positive WB detected inHepG2 cells, HuH-7 cells, human placenta
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 82982-5-RR is AFP Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name alpha-fetoprotein
Calculated molecular weight 69 kDa
Observed molecular weight68-72 kDa
GenBank accession numberBC027881
Gene symbol AFP
Gene ID (NCBI) 174
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AFP (Alpha-fetoprotein) is a major plasma protein in the fetus and its concentration is very low in the adult (PMID:24120489). AFP can be detected at abnormally high concentrations in hepatocellular carcinomas as well as in the plasma and ascitic fluid of adults with hepatoma, indicating that AFP can serve as a tumor marker (PMID: 18669658). AFP is also a glycosylated protein and based on its binding capability to lectin Lens Culinaris Agglutinin (LCA), and total AFP can be separated into three different glycoforms, AFP-L1, AFP-L2, and AFP-L3. Core-fucosylated form of AFP (AFP-L3) is a more specific indicator than total AFP for HCC (PMID: 33128033, 35458505)

Protocols

Product Specific Protocols
WB protocol for AFP antibody 82982-5-RRDownload protocol
IF protocol for AFP antibody 82982-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols