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  • Featured Product
  • KD/KO Validated

AGBL3 Polyclonal antibody

AGBL3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16990-1-AP

Synonyms

AGBL3, ATP/GTP binding protein like 3, CBPC3, CCP3

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using AGBL3 Polyclonal antibody (16990-1-AP)

    WB analysis of mouse liver using 16990-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 16990-1-AP (AGBL3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using AGBL3 Polyclonal antibody (16990-1-AP)

    WB analysis of L02 using 16990-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 16990-1-AP (AGBL3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using AGBL3 Polyclonal antibody (16990-1-AP)

    IHC staining of human lung cancer using 16990-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16990-1-AP (AGBL3 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using AGBL3 Polyclonal antibody (16990-1-AP)

    IHC staining of human lung cancer using 16990-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16990-1-AP (AGBL3 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using AGBL3 Polyclonal antibody (16990-1-AP)

    IHC staining of human testis using 16990-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 16990-1-AP (AGBL3 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using AGBL3 Polyclonal antibody (16990-1-AP)

    IHC staining of human testis using 16990-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 16990-1-AP (AGBL3 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inmouse liver tissue, L02 cells
Positive IHC detected inhuman lung cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16990-1-AP targets AGBL3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AGBL3 fusion protein Ag10592 相同性解析による交差性が予測される生物種
Full Name ATP/GTP binding protein-like 3
Calculated molecular weight 73 kDa, 116 kDa
Observed molecular weight 73 kDa
GenBank accession numberBC030651
Gene symbol AGBL3
Gene ID (NCBI) 340351
RRIDAB_2878337
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AGBL3, also named as CCP3, is a metallocarboxypeptidase that may play a role in the processing of tubulin. AGBL3 has some isoforms with MW 107 kDA, 73 kDa, 116 kDa and 20 kDa.

Protocols

Product Specific Protocols
WB protocol for AGBL3 antibody 16990-1-APDownload protocol
IHC protocol for AGBL3 antibody 16990-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Exp Med

Glutamylation of deubiquitinase BAP1 controls self-renewal of hematopoietic stem cells and hematopoiesis.

Authors - Zhen Xiong
  • KO Validated
View Article