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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

AGER/RAGE Recombinant antibody

AGER/RAGE Recombinant Antibody for WB, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

rat

Applications

WB, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

240706F7

Cat no : 83759-4-RR

Synonyms

RAGE, AGER, Advanced glycosylation end product-specific receptor, 240706F7

Validation Data Gallery

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  • Western Blot (WB) analysis of rat lung tissue using AGER Recombinant antibody (83759-4-RR)

    WB analysis of rat lung using 83759-4-RR

    rat lung tissue were subjected to SDS PAGE followed by western blot with 83759-4-RR (RAGE antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using AGER Recombinant antibody (83759-4-RR)

    IF Staining of rat brain using 83759-4-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using AGER antibody (83759-4-RR, Clone: 240706F7 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using AGER Recombinant antibody (83759-4-RR)

    IF Staining of rat brain using 83759-4-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using AGER antibody (83759-4-RR, Clone: 240706F7 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83759-4-RR

    Affinity and Kinetic Characterization of 83759-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83759-4-RR against Mouse AGER were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inrat lung tissue
Positive IF-P detected inrat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83759-4-RR is AGER/RAGE Fusion Protein expressed in E. coli.

Tested Reactivity rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name advanced glycosylation end product-specific receptor
Calculated molecular weight43 kDa
Observed molecular weight45 kDa, 58 kDa
Gene symbol Ager
Gene ID (NCBI) 81722
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Advanced glycosylation end product-specific receptor (AGER, also known as RAGE) is a member of the immunoglobulin superfamily of cell surface receptors, which interacts with distinct families of ligands, mediating diverse functions in a broad array of cell types including cellular migration, proliferation, survival and apoptosis (PMID: 12645002; 17425919). It senses endogenous stress signals with a broad ligand repertoire including advanced glycation end products, S100 proteins, high-mobility group box 1 protein/HMGB1, amyloid beta/APP oligomers, nucleic acids, phospholipids and glycosaminoglycans (PMID: 19910580; 28627626). It interacts with distinct molecules implicated in homeostasis, development, inflammation, and certain diseases such as diabetes and Alzheimer's disease (PMID: 26253613; 31079281).

Protocols

Product Specific Protocols
WB protocol for AGER/RAGE antibody 83759-4-RRDownload protocol
IF protocol for AGER/RAGE antibody 83759-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols