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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AGER/RAGE Recombinant antibody (83759-5-RR)

    WB analysis using 83759-5-RR (same clone as 83759-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83759-5-RR (RAGE antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83759-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using AGER/RAGE Recombinant antibody (83759-5-RR)

    IHC staining of mouse lung using 83759-5-RR (same clone as 83759-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 83759-5-RR (RAGE antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83759-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using AGER/RAGE Recombinant antibody (83759-5-RR)

    IHC staining of mouse lung using 83759-5-RR (same clone as 83759-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 83759-5-RR (RAGE antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83759-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat lung tissue using AGER/RAGE Recombinant antibody (83759-5-RR)

    IHC staining of rat lung using 83759-5-RR (same clone as 83759-5-PBS)

    Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 83759-5-RR (AGER/RAGE antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83759-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat lung tissue using AGER/RAGE Recombinant antibody (83759-5-RR)

    IHC staining of rat lung using 83759-5-RR (same clone as 83759-5-PBS)

    Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 83759-5-RR (AGER/RAGE antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83759-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse lung tissue using AGER/RAGE Recombinant antibody (83759-5-RR)

    IF Staining of mouse lung using 83759-5-RR (same clone as 83759-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse lung tissue using AGER/RAGE antibody (83759-5-RR, Clone: 240706C3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83759-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse lung tissue using AGER/RAGE Recombinant antibody (83759-5-RR)

    IF Staining of mouse lung using 83759-5-RR (same clone as 83759-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse lung tissue using AGER/RAGE antibody (83759-5-RR, Clone: 240706C3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83759-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83759-5-RR

    Affinity and Kinetic Characterization of 83759-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83759-5-RR against Rat AGER/RAGE were performed. The affinity constant is 3.46 nM.

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Rat AGER/RAGE Recombinant antibody, PBS Only

AGER/RAGE Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 83759-5-PBS
Clone No.240706C3

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

RAGE, AGER, Advanced glycosylation end product-specific receptor, 240706C3

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83759-5-PBS targets AGER/RAGE in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name advanced glycosylation end product-specific receptor
Calculated molecular weight43 kDa
Observed molecular weight43 kDa
Gene Symbol Ager
Gene ID (NCBI) 81722
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ63495
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Advanced glycosylation end product-specific receptor (AGER, also known as RAGE) is a member of the immunoglobulin superfamily of cell surface receptors, which interacts with distinct families of ligands, mediating diverse functions in a broad array of cell types including cellular migration, proliferation, survival and apoptosis (PMID: 12645002; 17425919). It senses endogenous stress signals with a broad ligand repertoire including advanced glycation end products, S100 proteins, high-mobility group box 1 protein/HMGB1, amyloid beta/APP oligomers, nucleic acids, phospholipids and glycosaminoglycans (PMID: 19910580; 28627626). It interacts with distinct molecules implicated in homeostasis, development, inflammation, and certain diseases such as diabetes and Alzheimer's disease (PMID: 26253613; 31079281).