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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

AGGF1 Recombinant antibody

AGGF1 Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

242264F4

Cat no : 84715-4-RR

Synonyms

HSU84971, GPATCH7, GPATC7, G patch domain-containing protein 7, Angiogenic factor VG5Q

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using AGGF1 Recombinant antibody (84715-4-RR)

    IF Staining of HeLa using 84715-4-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using AGGF1 antibody (84715-4-RR, Clone: 242264F4 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84715-4-RR

    Affinity and Kinetic Characterization of 84715-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84715-4-RR against Human AGGF1 were performed. The affinity constant is 7.15 nM.

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Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84715-4-RR targets AGGF1 in IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen AGGF1 fusion protein Ag2497 相同性解析による交差性が予測される生物種
Full Name angiogenic factor with G patch and FHA domains 1
Calculated molecular weight 714 aa, 81 kDa
GenBank accession numberBC029382
Gene symbol AGGF1
Gene ID (NCBI) 55109
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The angiogenic factor gene, AGGF1 (or VG5Q), is identified as a candidate susceptibility gene for Klippel-Trenaunay syndrome (KTS) which is a severe congenital disorder characterized by capillary malformations, venous malformations or varicose veins, and hypertrophy of the affected tissues. AGGF1 protein can bind to endothelial cells and promote cell proliferation. AGGF1 shows strong expression in blood vessels and is secreted as vessel formation is initiated. Regulation of AGGF1 by GATA1 may play roles in endothelial cell biology and angiogenesis.

Protocols

Product Specific Protocols
IF protocol for AGGF1 antibody 84715-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols