Validation Data Gallery

Filter: AllWBIHC

WB analysis using 16582-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16582-1-AP (AGL antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 16582-1-AP
WB result of AGL antibody (16582-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AGL transfected HEK-293 cells.
IHC staining of human heart using 16582-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16582-1-AP (AGL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human heart using 16582-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16582-1-AP (AGL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

AGL Polyclonal antibody

AGL Polyclonal Antibody for WB, IHC, ELISA

Cat No. 16582-1-AP

Publications(6)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

4-alpha-glucanotransferase, Amylo-1,6-glucosidase, Amylo-alpha-1,6-glucosidase, Dextrin 6-alpha-D-glucosidase, Glycogen debrancher

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (6)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	Jurkat cells, HEK-293 cells, mouse heart tissue, rat heart tissue
Positive IHC detected in	human heart tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 6 publications below

Product Information

16582-1-AP targets AGL in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	AGL fusion protein Ag9962 相同性解析による交差性が予測される生物種
Full Name	amylo-1, 6-glucosidase, 4-alpha-glucanotransferase
Calculated molecular weight	1532 aa, 175 kDa
Observed molecular weight	165 kDa
GenBank accession number	BC078663
Gene Symbol	AGL
Gene ID (NCBI)	178
RRID	AB_2878283
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P35573
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Amylo-alpha-1-6-glucosidase-4-alpha-glucanotransferase (AGL) (also known as glycogen debranching enzyme) is a critical bifunctional enzyme involved in glycogen metabolism.

Protocols

Product Specific Protocols
WB protocol for AGL antibody 16582-1-AP	Download protocol
IHC protocol for AGL antibody 16582-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	J Clin Invest A functional mini-GDE transgene corrects impairment in models of glycogen storage disease type III Authors - Antoine Gardin View Article
rat	WB	Carbohydr Polym Liver fibrosis alters the molecular structures of hepatic glycogen Authors - Yujun Wan View Article
human	WB	iScience MYCT1 alters the glycogen shunt by regulating selective translation of RACK1-mediated enzymes. Authors - Dong-Xue Ding View Article
rat	WB	PLoS One A novel genetic model provides a unique perspective on the relationship between postexercise glycogen concentration and increases in the abundance of key metabolic proteins after acute exercise Authors - Seong Eun Kwak View Article
mouse	WB	JCI Insight Synergism of dual AAV gene therapy and rapamycin rescues GSDIII phenotype in muscle and liver Authors - Louisa Jauze View Article
human	WB	Stem Cell Res Generation of three induced pluripotent stem cell lines from patients with glycogen storage disease type III Authors - Lucille Rossiaud View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 16582-1-AP

WB analysis of HEK-293 using 16582-1-AP

IHC staining of human heart using 16582-1-AP

IHC staining of human heart using 16582-1-AP

AGL Polyclonal antibody

AGL Polyclonal Antibody for WB, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

A functional mini-GDE transgene corrects impairment in models of glycogen storage disease type III

Liver fibrosis alters the molecular structures of hepatic glycogen

MYCT1 alters the glycogen shunt by regulating selective translation of RACK1-mediated enzymes.

A novel genetic model provides a unique perspective on the relationship between postexercise glycogen concentration and increases in the abundance of key metabolic proteins after acute exercise

Synergism of dual AAV gene therapy and rapamycin rescues GSDIII phenotype in muscle and liver

Generation of three induced pluripotent stem cell lines from patients with glycogen storage disease type III