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AGL Polyclonal antibody
AGL Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 16582-1-AP
Synonyms
Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | Jurkat cells, HEK-293 cells, mouse heart tissue, rat heart tissue |
| Positive IHC detected in | human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 6 publications below |
Product Information
16582-1-AP targets AGL in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | AGL fusion protein Ag9962 相同性解析による交差性が予測される生物種 |
| Full Name | amylo-1, 6-glucosidase, 4-alpha-glucanotransferase |
| Calculated molecular weight | 1532 aa, 175 kDa |
| Observed molecular weight | 165 kDa |
| GenBank accession number | BC078663 |
| Gene symbol | AGL |
| Gene ID (NCBI) | 178 |
| RRID | AB_2878283 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for AGL antibody 16582-1-AP | Download protocol |
| IHC protocol for AGL antibody 16582-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Clin Invest A functional mini-GDE transgene corrects impairment in models of glycogen storage disease type III | ||
iScience MYCT1 alters the glycogen shunt by regulating selective translation of RACK1-mediated enzymes. | ||
PLoS One A novel genetic model provides a unique perspective on the relationship between postexercise glycogen concentration and increases in the abundance of key metabolic proteins after acute exercise | ||
JCI Insight Synergism of dual AAV gene therapy and rapamycin rescues GSDIII phenotype in muscle and liver | ||
Stem Cell Res Generation of three induced pluripotent stem cell lines from patients with glycogen storage disease type III |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Jeremy (Verified Customer) (09-09-2019) | Highly sensitive for the detection of Agl protein in different tissue including Heart, Diaphragm and different muscles
 |
