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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AGPAT4 Polyclonal antibody (16562-1-AP)

    WB analysis using 16562-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16562-1-AP (AGPAT4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using AGPAT4 Polyclonal antibody (16562-1-AP)

    WB analysis using 16562-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16562-1-AP (AGPAT4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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AGPAT4 Polyclonal antibody

AGPAT4 Polyclonal Antibody for WB, ELISA
Cat No. 16562-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

1-AGP acyltransferase 4, 1-acyl-sn-glycerol-3-phosphate acyltransferase delta, 1-acylglycerol-3-phosphate O-acyltransferase 4, 1 AGPAT4, 1 AGPAT 4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, U-251 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16562-1-AP targets AGPAT4 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AGPAT4 fusion protein Ag9834 相同性解析による交差性が予測される生物種
Full Name 1-acylglycerol-3-phosphate O-acyltransferase 4 (lysophosphatidic acid acyltransferase, delta)
Calculated molecular weight 378 aa, 44 kDa
Observed molecular weight44 kDa
GenBank accession numberBC020209
Gene Symbol AGPAT4
Gene ID (NCBI) 56895
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NRZ5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AGPAT4 (Acylglycerophosphate acyltransferase 4), also known as LPAATδ (Lysophosphatidic acid acyltransferase delta), is a protein expressed mainly in brain and in muscle. AGPAT4 is characterized as a mitochondrial lysophosphatidic acid acyltransferase that regulates brain levels of phosphatidylcholine (PC), phosphatidylethanolamine (PE), and phosphatidylinositol (PI) (PMID: 28807933). AGPAT4 is relatively abundant in murine renal white adipose tissue (WAT) and has been linked to the regulation of TAG production, the differentiation of adipocytes, and the modulation of various pathologies including lipodystrophy, diabetes, and hepatic steatosis (PMID: 28814640).

Protocols

Product Specific Protocols
WB protocol for AGPAT4 antibody 16562-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols