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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AGPAT6 Polyclonal antibody (16762-1-AP)

    WB analysis using 16762-1-AP (same clone as 16762-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16762-1-AP (AGPAT6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16762-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using AGPAT6 Polyclonal antibody (16762-1-AP)

    WB analysis of mouse testis using 16762-1-AP (same clone as 16762-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 16762-1-AP (AGPAT6 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16762-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using AGPAT6 Polyclonal antibody (16762-1-AP)

    IP experiment of mouse brain using 16762-1-AP (same clone as 16762-1-PBS)

    IP result of anti-AGPAT6 (IP:16762-1-AP, 4ug; Detection:16762-1-AP 1:1000) with mouse brain tissue lysate 2640ug. This data was developed using the same antibody clone with 16762-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using AGPAT6 Polyclonal antibody (16762-1-AP)

    IHC staining of human testis using 16762-1-AP (same clone as 16762-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 16762-1-AP (AGPAT6 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 16762-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using AGPAT6 Polyclonal antibody (16762-1-AP)

    IHC staining of human testis using 16762-1-AP (same clone as 16762-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 16762-1-AP (AGPAT6 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 16762-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin tissue using AGPAT6 Polyclonal antibody (16762-1-AP)

    IHC staining of human skin using 16762-1-AP (same clone as 16762-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin using 16762-1-AP (AGPAT6 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 16762-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin tissue using AGPAT6 Polyclonal antibody (16762-1-AP)

    IHC staining of human skin using 16762-1-AP (same clone as 16762-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin using 16762-1-AP (AGPAT6 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 16762-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using AGPAT6 Polyclonal antibody (16762-1-AP)

    IHC staining of human kidney using 16762-1-AP (same clone as 16762-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 16762-1-AP (AGPAT6 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 16762-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using AGPAT6 Polyclonal antibody (16762-1-AP)

    IHC staining of human kidney using 16762-1-AP (same clone as 16762-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 16762-1-AP (AGPAT6 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 16762-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tissue using AGPAT6 Polyclonal antibody (16762-1-AP)

    IHC staining of human ovary using 16762-1-AP (same clone as 16762-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary using 16762-1-AP (AGPAT6 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 16762-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tissue using AGPAT6 Polyclonal antibody (16762-1-AP)

    IHC staining of human ovary using 16762-1-AP (same clone as 16762-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary using 16762-1-AP (AGPAT6 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 16762-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using AGPAT6 Polyclonal antibody (16762-1-AP)

    IHC staining of human liver using 16762-1-AP (same clone as 16762-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 16762-1-AP (AGPAT6 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 16762-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using AGPAT6 Polyclonal antibody (16762-1-AP)

    IHC staining of human liver using 16762-1-AP (same clone as 16762-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 16762-1-AP (AGPAT6 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 16762-1-PBS in a different storage buffer formulation.

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AGPAT6 Polyclonal antibody, PBS Only

AGPAT6 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 16762-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16762-1-PBS targets AGPAT6 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AGPAT6 fusion protein Ag10294 相同性解析による交差性が予測される生物種
Full Name 1-acylglycerol-3-phosphate O-acyltransferase 6 (lysophosphatidic acid acyltransferase, zeta)
Calculated molecular weight 456 aa, 52 kDa
Observed molecular weight52 kDa
GenBank accession numberBC051377
Gene Symbol AGPAT6
Gene ID (NCBI) 137964
RRIDAB_1850878
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86UL3
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

AGPAT6 is a member of the 1-acylglycerol-3-phosphate O-acyltransferase (AGPAT) family. AGPAT6 is expressed in brown adipose tissue and mammary epithelium as well as many other tissues. Adeficiency of AGPAT6 in mice results in resistance to dietinduced and genetic forms of obesity.