• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of pig stomach tissue using AGR2 Monoclonal antibody (66768-1-Ig)

    WB analysis of pig stomach using 66768-1-Ig (same clone as 66768-1-PBS)

    pig stomach tissue were subjected to SDS PAGE followed by western blot with 66768-1-Ig (AGR2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66768-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of T-47D cells using AGR2 Monoclonal antibody (66768-1-Ig)

    WB analysis of T-47D using 66768-1-Ig (same clone as 66768-1-PBS)

    T-47D cells were subjected to SDS PAGE followed by western blot with 66768-1-Ig (AGR2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66768-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HT-29 cells using AGR2 Monoclonal antibody (66768-1-Ig)

    WB analysis of HT-29 using 66768-1-Ig (same clone as 66768-1-PBS)

    HT-29 cells were subjected to SDS PAGE followed by western blot with 66768-1-Ig (AGR2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66768-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using AGR2 Monoclonal antibody (66768-1-Ig)

    IHC staining of human breast cancer using 66768-1-Ig (same clone as 66768-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66768-1-Ig (AGR2 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66768-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using AGR2 Monoclonal antibody (66768-1-Ig)

    IHC staining of human breast cancer using 66768-1-Ig (same clone as 66768-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66768-1-Ig (AGR2 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66768-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human colon cancer tissue using AGR2 Monoclonal antibody (66768-1-Ig)

    IF Staining of human colon cancer using 66768-1-Ig (same clone as 66768-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using AGR2 antibody (66768-1-Ig, Clone: 1A8A8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66768-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human colon cancer tissue using AGR2 Monoclonal antibody (66768-1-Ig)

    IF Staining of human colon cancer using 66768-1-Ig (same clone as 66768-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using AGR2 antibody (66768-1-Ig, Clone: 1A8A8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66768-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using AGR2 Monoclonal antibody (66768-1-Ig)

    IF Staining of A549 using 66768-1-Ig (same clone as 66768-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using AGR2 antibody (66768-1-Ig, Clone: 1A8A8 ) at dilution of 1:1500 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004). This data was developed using the same antibody clone with 66768-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HT-29 cells using AGR2 Monoclonal antibody (66768-1-Ig)

    IF Staining of HT-29 using 66768-1-Ig (same clone as 66768-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HT-29 cells using AGR2 antibody (66768-1-Ig, Clone: 1A8A8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66768-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HT-29 cells using AGR2 Monoclonal antibody (66768-1-Ig)

    FC experiment of HT-29 using 66768-1-Ig (same clone as 66768-1-PBS)

    1X10^6 HT-29 cells were intracellularly stained with 0.2 ug Anti-Human AGR2 (66768-1-Ig, Clone:1A8A8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), and 0.2 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100. This data was developed using the same antibody clone with 66768-1-PBS in a different storage buffer formulation.

☆ 3/31（月）まで！二次抗体 & FlexAbleが30%オフ！ >> 免疫アッセイサポートキャンペーン

AGR2 Monoclonal antibody, PBS Only

AGR2 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA
Cat No. 66768-1-PBS
Clone No.1A8A8

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

Synonyms

GOB 4, AGR 2, AG-2, AG2, AG 2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66768-1-PBS targets AGR2 in WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen AGR2 fusion protein Ag2919 相同性解析による交差性が予測される生物種
Full Name anterior gradient homolog 2 (Xenopus laevis)
Calculated molecular weight 175 aa, 20 kDa
Observed molecular weight 17 kDa
GenBank accession numberBC015503
Gene Symbol AGR2
Gene ID (NCBI) 10551
RRIDAB_2882114
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO95994
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

AGR2, also named AG2 or HPC8, encodes anterior gradient protein 2 homolog which belongs to the AGR family. It is a secreted protein localized in endoplasmic reticulum. AGR2 plays roles in MUC2 post-transcriptional synthesis,secretion and production of mucus by intestinal cells. AGR2 was significantly elevated in the pancreatic juice from patients with pre-malignant conditions as well as pancreatic cancer compared to control pancreatic juice samples. AGR2 levels in pancreatic juice could potentially be used to aide in assessment of high-risk patients undergoing endoscopic procedures.