AGR3 Polyclonal antibody

Name: AGR3 Polyclonal antibody
Brand: Proteintech
SKU: 11967-1-AP

AGR3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 11967-1-AP

Synonyms

hAG 3, Anterior gradient protein 3, Anterior gradient 3 homolog, AG-3, AG3

Validation Data Gallery

Filter:

AllWBIHCIF/ICC

WB analysis of SKOV-3 using 11967-1-AP
SKOV-3 cells were subjected to SDS PAGE followed by western blot with 11967-1-AP (AGR3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of SKOV-3 using 11967-1-AP
SKOV-3 cells were subjected to SDS PAGE followed by western blot with 11967-1-AP (AGR3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 11967-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 11967-1-AP (AGR3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 11967-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 11967-1-AP (AGR3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of human breast cancer using 11967-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11967-1-AP (AGR3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 11967-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11967-1-AP (AGR3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of SKOV-3 using 11967-1-AP
Immunofluorescent analysis of SKOV-3 cells using 11967-1-AP (AGR3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 11967-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	SKOV-3 cells, MCF-7 cells
Positive IHC detected in	human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	SKOV-3 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 2 publications below
IHC	See 2 publications below
IF	See 4 publications below

Product Information

11967-1-AP targets AGR3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, chicken
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	AGR3 fusion protein Ag2577 相同性解析による交差性が予測される生物種
Full Name	anterior gradient homolog 3 (Xenopus laevis)
Calculated molecular weight	166 aa, 19 kDa
Observed molecular weight	19-25 kDa
GenBank accession number	BC058284
Gene symbol	AGR3
Gene ID (NCBI)	155465
RRID	AB_2877809
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for AGR3 antibody 11967-1-AP	Download protocol
IHC protocol for AGR3 antibody 11967-1-AP	Download protocol
IF protocol for AGR3 antibody 11967-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
chicken	IF	Elife Single-cell proteomics reveals changes in expression during hair-cell development. Authors - Ying Zhu View Article
human	WB,IHC	Oncotarget Expression of protein disulfide isomerase family members correlates with tumor progression and patient survival in ovarian cancer. Authors - Soma Samanta View Article
human	IHC, IF, WB	Front Pharmacol AGR3 Regulates Airway Epithelial Junctions in Patients with Frequent Exacerbations of COPD. Authors - Rui Ye View Article
chicken	IF	iScience Stepwise fate conversion of supporting cells to sensory hair cells in the chick auditory epithelium Authors - Mami Matsunaga View Article
human	IF	J Transl Med Single-cell RNA sequencing reveals small extracellular vesicles derived from malignant cells that contribute to angiogenesis in human breast cancers Authors - Youxue Zhang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

AGR3 Polyclonal antibody

AGR3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis of SKOV-3 using 11967-1-AP

WB analysis of SKOV-3 using 11967-1-AP

WB analysis of MCF-7 using 11967-1-AP

WB analysis of MCF-7 using 11967-1-AP

IHC staining of human breast cancer using 11967-1-AP

IHC staining of human breast cancer using 11967-1-AP

IF Staining of SKOV-3 using 11967-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Single-cell proteomics reveals changes in expression during hair-cell development.

Expression of protein disulfide isomerase family members correlates with tumor progression and patient survival in ovarian cancer.

AGR3 Regulates Airway Epithelial Junctions in Patients with Frequent Exacerbations of COPD.

Stepwise fate conversion of supporting cells to sensory hair cells in the chick auditory epithelium

Single-cell RNA sequencing reveals small extracellular vesicles derived from malignant cells that contribute to angiogenesis in human breast cancers