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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AGRN Polyclonal antibody (20692-1-AP)

    WB analysis using 20692-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20692-1-AP (AGRN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of Saos-2 cells using AGRN Polyclonal antibody (20692-1-AP)

    IF Staining of Saos-2 using 20692-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed Saos-2 cells using AGRN antibody (20692-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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AGRN Polyclonal antibody

AGRN Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 20692-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Agrin N-terminal 110 kDa subunit, Agrin C-terminal 90 kDa fragment, Agrin C-terminal 22 kDa fragment, Agrin C-terminal 110 kDa subunit, Agrin

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inDU 145 cells
Positive IF/ICC detected inSaos-2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20692-1-AP targets AGRN in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name agrin
Calculated molecular weight 215 kDa
Observed molecular weight225 kDa, 97 kDa
GenBank accession numberNM_198576
Gene Symbol AGRN
Gene ID (NCBI) 375790
RRIDAB_3085612
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00468
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Agrin, a large heparan sulfate proteoglycan, is expressed in neuronal, as well as nonneuronal tissues (PMID:12671652). Nerve-derived agrin has been demonstrated to play an essential role in development and maintenance of the neuromuscular junction (PMID:16386415). The agrin gene encodes a protein of more than 2000 amino acids with predicted molecular weight of 225 kDa but can display as 600 kDa protein due to extensive N-terminal glycosylation (PMID:12671652). Neurotrypsin cleaves the proteoglycan agrin at two homologous, highly conserved sites, liberating a middle 90-kDa and a C-terminal 22-kDa fragment from the membrane or ECM-bound N-terminal moiety of agrin (PMID:18230682).

Protocols

Product Specific Protocols
WB protocol for AGRN antibody 20692-1-APDownload protocol
IF protocol for AGRN antibody 20692-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols