- Featured Product
- KD/KO Validated
CCP1 Polyclonal antibody
CCP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 14067-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, A549 cells, mouse testis tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human testis tissue, human heart tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 13 publications below |
| IHC | See 5 publications below |
| IF | See 7 publications below |
| IP | See 1 publications below |
Product Information
14067-1-AP targets CCP1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CCP1 fusion protein Ag5122 相同性解析による交差性が予測される生物種 |
| Full Name | ATP/GTP binding protein 1 |
| Calculated molecular weight | 138 kDa |
| Observed molecular weight | 138 kDa |
| GenBank accession number | BC060815 |
| Gene symbol | CCP1 |
| Gene ID (NCBI) | 23287 |
| RRID | AB_2225559 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
AGTPBP1, also known as CCP1 (cytosolic carboxy-peptidase 1), or Nna1, belongs to the peptidase M14 family. AGTPBP1 is a zinc carboxypeptidase that contains nuclear localization signals and an ATP/GTP-binding motiff. Thus, it may contribute to nuclear signaling events in differentiating and regenerating neurons. It serves important functions in the nervous system, and AGTPBP1 mutations may cause Purkinje cell degeneration (pcd) phenotypes.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CCP1 antibody 14067-1-AP | Download protocol |
| IHC protocol for CCP1 antibody 14067-1-AP | Download protocol |
| IF protocol for CCP1 antibody 14067-1-AP | Download protocol |
| IP protocol for CCP1 antibody 14067-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Klf4 glutamylation is required for cell reprogramming and early embryonic development in mice.
| ||
Nat Commun AMPK activation promotes lipid droplet dispersion on detyrosinated microtubules to increase mitochondrial fatty acid oxidation. | ||
Proc Natl Acad Sci U S A Pathogenic role of delta 2 tubulin in bortezomib-induced peripheral neuropathy.
| ||
J Cell Biol CCP1 promotes mitochondrial fusion and motility to prevent Purkinje cell neuron loss in pcd mice. |
