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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using AGXT Polyclonal antibody (22394-1-AP)

    WB analysis of mouse liver using 22394-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 22394-1-AP (AGXT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using AGXT Polyclonal antibody (22394-1-AP)

    WB analysis of mouse lung using 22394-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 22394-1-AP (AGXT antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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AGXT Polyclonal antibody

AGXT Polyclonal Antibody for WB, ELISA
Cat No. 22394-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, ELISA

Synonyms

EC:2.6.1.51, EC:2.6.1.44, Alanine--glyoxylate aminotransferase, AGXT1, AGT1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse liver tissue, mouse lung tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

22394-1-AP targets AGXT in WB, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AGXT fusion protein Ag18062 相同性解析による交差性が予測される生物種
Full Name alanine-glyoxylate aminotransferase
Calculated molecular weight 392 aa, 43 kDa
Observed molecular weight 43-45 kDa
GenBank accession numberBC132819
Gene Symbol AGXT
Gene ID (NCBI) 189
RRIDAB_2879098
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP21549
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AGXT (AGT) is a key enzyme in glyoxylate detoxification. Mutations of AGXT lead to primary hyperoxaluria type I, a rare metabolic disease that is characterized by deficient AGXT, and excessive hepatic oxalate production that leads to renal failure.

Protocols

Product Specific Protocols
WB protocol for AGXT antibody 22394-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Nucleic Acid Ther

Systemic Alanine Glyoxylate Aminotransferase Messenger RNA Improves Glyoxylate Metabolism in a Mouse Model of Primary Hyperoxaluria Type 1.

Authors - Anjli Kukreja
View Article