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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

AGXT Monoclonal antibody

AGXT Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3E12H1

Cat no : 67402-1-Ig

Synonyms

AGT, AGT1, AGXT, AGXT1, PH1, SPAT, SPT, TLH6

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AGXT Monoclonal antibody (67402-1-Ig)

    WB analysis using 67402-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67402-1-Ig (AGXT antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NCI-H1299 cells using AGXT Monoclonal antibody (67402-1-Ig)

    WB analysis of NCI-H1299 using 67402-1-Ig

    NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 67402-1-Ig (AGXT antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using AGXT Monoclonal antibody (67402-1-Ig)

    WB analysis of A549 using 67402-1-Ig

    A549 cells were subjected to SDS PAGE followed by western blot with 67402-1-Ig (AGXT antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using AGXT Monoclonal antibody (67402-1-Ig)

    IHC staining of human liver cancer using 67402-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67402-1-Ig (AGXT antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using AGXT Monoclonal antibody (67402-1-Ig)

    IHC staining of human kidney using 67402-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67402-1-Ig (AGXT antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHepG2 cells, NCI-H1299 cells, A549 cells, L02 cells, HuH-7 cells, SMMC-7721 cells
Positive IHC detected inhuman liver cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67402-1-Ig targets AGXT in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen AGXT fusion protein Ag19188 相同性解析による交差性が予測される生物種
Full Name alanine-glyoxylate aminotransferase
Calculated molecular weight 392 aa, 43 kDa
Observed molecular weight 43 kDa
GenBank accession numberBC132819
Gene symbol AGXT
Gene ID (NCBI) 189
RRIDAB_2882644
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AGXT (AGT) is a key enzyme in glyoxylate detoxification. Mutations of AGXT lead to primary hyperoxaluria type I, a rare metabolic disease which is characterized by deficient AGXT, excessive hepatic oxalate production that leads to renal failure.

Protocols

Product Specific Protocols
WB protocol for AGXT antibody 67402-1-IgDownload protocol
IHC protocol for AGXT antibody 67402-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols