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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AGXT Recombinant antibody (83385-6-RR)

    WB analysis using 83385-6-RR (same clone as 83385-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83385-6-RR (AGXT antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83385-6-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using AGXT Recombinant antibody (83385-6-RR)

    WB analysis using 83385-6-RR (same clone as 83385-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83385-6-RR (AGXT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83385-6-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using AGXT Recombinant antibody (83385-6-RR)

    IF Staining of HeLa using 83385-6-RR (same clone as 83385-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using AGXT antibody (83385-6-RR, Clone: 240305E7 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83385-6-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using AGXT Recombinant antibody (83385-6-RR)

    FC experiment of HepG2 using 83385-6-RR (same clone as 83385-6-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug AGXT Recombinant antibody (83385-6-RR, Clone:240305E7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83385-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83385-6-RR

    Affinity and Kinetic Characterization of 83385-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83385-6-RR against Human AGXT were performed. The affinity constant is 0.226 nM.

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AGXT Recombinant antibody, PBS Only

AGXT Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83385-6-PBS
Clone No.240305E7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

EC:2.6.1.44, Alanine--glyoxylate aminotransferase, AGXT1, AGT1, AGT

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83385-6-PBS targets AGXT in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen AGXT fusion protein Ag18062 相同性解析による交差性が予測される生物種
Full Name alanine-glyoxylate aminotransferase
Calculated molecular weight 392 aa, 43 kDa
GenBank accession numberBC132819
Gene Symbol AGXT
Gene ID (NCBI) 189
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP21549
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.