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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse liver tissue using AGXT2L1 Recombinant antibody (83984-5-RR)

    IHC staining of mouse liver using 83984-5-RR (same clone as 83984-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83984-5-RR (AGXT2L1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83984-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using AGXT2L1 Recombinant antibody (83984-5-RR)

    IHC staining of mouse liver using 83984-5-RR (same clone as 83984-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83984-5-RR (AGXT2L1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83984-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using AGXT2L1 Recombinant antibody (83984-5-RR)

    IF Staining of U2OS using 83984-5-RR (same clone as 83984-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using AGXT2L1 antibody (83984-5-RR, Clone: 241119H5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83984-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83984-5-RR

    Affinity and Kinetic Characterization of 83984-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83984-5-RR against Human AGXT2L1 were performed. The affinity constant is 1.77 nM.

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AGXT2L1 Recombinant antibody, PBS Only

AGXT2L1 Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, ELISA
Cat No. 83984-5-PBS
Clone No.241119H5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, ELISA

Synonyms

ETNPPL, Ethanolamine-phosphate phospho-lyase, EC:4.2.3.2, Alanine--glyoxylate aminotransferase 2-like 1, 241119H5

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83984-5-PBS targets AGXT2L1 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen AGXT2L1 fusion protein Ag10584 相同性解析による交差性が予測される生物種
Full Name alanine-glyoxylate aminotransferase 2-like 1
Calculated molecular weight 499 aa, 56 kDa
GenBank accession numberBC022526
Gene Symbol AGXT2L1
Gene ID (NCBI) 64850
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ8TBG4
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Alanine-glyoxylate aminotransferase 2-like 1 (AGXT2L1), also called ethanolamine phosphate phospholyase (ETNPPL), is a brain enzyme, so-named after its similarity to AGXT2 (36% sequence identity), that may be involved in the pathogenesis of severe neuropsychiatric disturbances(PMID: 23761375). AGXT2L1 is significantly down-regulated in cancerous tissues and may be a tumor suppressor(PMID: 26294768,