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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AHCY Monoclonal antibody (66019-1-Ig)

    WB analysis using 66019-1-Ig (same clone as 66019-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66019-1-Ig (AHCY antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66019-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human testis tissue using AHCY Monoclonal antibody (66019-1-Ig)

    WB analysis of human testis using 66019-1-Ig (same clone as 66019-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 66019-1-Ig (AHCY antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66019-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using AHCY Monoclonal antibody (66019-1-Ig)

    WB analysis of HeLa using 66019-1-Ig (same clone as 66019-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66019-1-Ig (AHCY antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66019-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using AHCY Monoclonal antibody (66019-1-Ig)

    WB analysis of MCF-7 using 66019-1-Ig (same clone as 66019-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 66019-1-Ig (AHCY antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66019-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of L02 cells using AHCY Monoclonal antibody (66019-1-Ig)

    WB analysis of L02 using 66019-1-Ig (same clone as 66019-1-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 66019-1-Ig (AHCY antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66019-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using AHCY Monoclonal antibody (66019-1-Ig)

    IHC staining of human colon cancer using 66019-1-Ig (same clone as 66019-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66019-1-Ig (AHCY antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66019-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using AHCY Monoclonal antibody (66019-1-Ig)

    IHC staining of human colon cancer using 66019-1-Ig (same clone as 66019-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66019-1-Ig (AHCY antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66019-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using AHCY Monoclonal antibody (66019-1-Ig)

    IHC staining of human testis using 66019-1-Ig (same clone as 66019-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 66019-1-Ig(AHCY antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66019-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using AHCY Monoclonal antibody (66019-1-Ig)

    IHC staining of human testis using 66019-1-Ig (same clone as 66019-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 66019-1-Ig(AHCY antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66019-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using AHCY Monoclonal antibody (66019-1-Ig)

    IHC staining of human placenta using 66019-1-Ig (same clone as 66019-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta using 66019-1-Ig(AHCY antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66019-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using AHCY Monoclonal antibody (66019-1-Ig)

    IHC staining of human placenta using 66019-1-Ig (same clone as 66019-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta using 66019-1-Ig(AHCY antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66019-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using AHCY Monoclonal antibody (66019-1-Ig)

    IF Staining of HeLa using 66019-1-Ig (same clone as 66019-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using AHCY antibody (66019-1-Ig, Clone: 1F9B10 ) at dilution of 1:4000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66019-1-PBS in a different storage buffer formulation.

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AHCY Monoclonal antibody, PBS Only

AHCY Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66019-1-PBS
Clone No.1F9B10

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Adenosylhomocysteinase, AdoHcyase, AHCY, S adenosylhomocysteine hydrolase, SAHH

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66019-1-PBS targets AHCY in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen AHCY fusion protein Ag18036 相同性解析による交差性が予測される生物種
Full Name S-adenosylhomocysteine hydrolase
Calculated molecular weight 48 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC011606
Gene Symbol AHCY
Gene ID (NCBI) 191
RRIDAB_11044194
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP23526
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

AHCY, also named as SAHH, belongs to the adenosylhomocysteinase family. AHCY is a competitive inhibitor of S-adenosyl-L-methionine-dependent methyl transferase reactions; therefore AHCY may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine. AHCY catalyzes the reversible hydrolysis of S-adenosylhomocysteine to adenosine and L-homocysteine.(PMID:21220475).