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AHCYL1 Polyclonal antibody

Name: AHCYL1 Polyclonal antibody
Brand: Proteintech
SKU: 10658-3-AP

AHCYL1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 10658-3-AP

Synonyms

S-adenosylhomocysteine hydrolase-like protein 1, IRBIT, IP(3)Rs binding protein released with IP(3), DC-expressed AHCY-like molecule, DCAL

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis of HeLa using 10658-3-AP
WB result of AHCYL1 antibody (10658-3-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AHCYL1 transfected HeLa cells.
WB analysis of Jurkat using 10658-3-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 10658-3-AP (AHCYL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse thymus using 10658-3-AP
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 10658-3-AP (AHCYL1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10658-3-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10658-3-AP (AHCYL1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 10658-3-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 10658-3-AP (AHCYL1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 10658-3-AP
IP result of anti-AHCYL1 (IP:10658-3-AP, 4ug; Detection:10658-3-AP 1:1000) with HeLa cells lysate 2400ug.
IHC staining of human liver cancer using 10658-3-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10658-3-AP (AHCYL1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 10658-3-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10658-3-AP (AHCYL1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 10658-3-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using AHCYL1 antibody (10658-3-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 10658-3-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	Jurkat cells, HeLa cells, mouse thymus tissue
Positive IP detected in	HeLa cells
Positive IHC detected in	human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:100-1:400
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 19 publications below
IHC	See 1 publications below
IP	See 2 publications below

Product Information

10658-3-AP targets AHCYL1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, chicken
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	AHCYL1 fusion protein Ag1017 相同性解析による交差性が予測される生物種
Full Name	S-adenosylhomocysteine hydrolase-like 1
Calculated molecular weight	65 kDa
Observed molecular weight	60 kDa
GenBank accession number	BC007576
Gene symbol	AHCYL1
Gene ID (NCBI)	10768
RRID	AB_2226013
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

S-adenosylhomocysteine hydrolase-like protein 1 (AHCYL1), also known as IP3 receptor-binding protein released with IP3 (IRBIT), regulates IP3-induced Ca2+ release into the cytoplasm of cells(PMID:23145124). This protein is also named as DCAL, XPVKONA and belongs to the adenosylhomocysteinase family. AHCYL1 mRNA and protein are most abundant in the glandular epithelium of adenocarcinoma of cancerous, but not normal and AHCYL1 protein is predominantly in the nucleus(PMID:22826361). It has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for AHCYL1 antibody 10658-3-AP	Download protocol
IHC protocol for AHCYL1 antibody 10658-3-AP	Download protocol
IF protocol for AHCYL1 antibody 10658-3-AP	Download protocol
IP protocol for AHCYL1 antibody 10658-3-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
pig	WB	J Agric Food Chem Maternal Betaine Supplementation during Gestation Enhances Expression of mtDNA-Encoded Genes through D-Loop DNA Hypomethylation in the Skeletal Muscle of Newborn Piglets. Authors - Yimin Jia View Article
rat	WB	J Agric Food Chem Mitotically stable modification of DNA methylation in IGF2/H19 imprinting control region is associated with activated hepatic IGF2 expression in offspring rats from betaine-supplemented dams. Authors - Shu Yang View Article
rat	WB	Mol Nutr Food Res Transgenerational Inheritance of Betaine-Induced Epigenetic Alterations in Estrogen-Responsive IGF-2/IGFBP2 Genes in Rat Hippocampus. Authors - Yang Yang View Article
rat	WB	Mol Nutr Food Res Maternal betaine supplementation in rats induces intergenerational changes in hepatic IGF-1 expression and DNA methylation. Authors - Nannan Zhao View Article
human	WB	Pharmaceutics Protective Role of IRBIT on Sodium Bicarbonate Cotransporter-n1 for Migratory Cancer Cells. Authors - Soyoung Hwang View Article
chicken	WB	J Nutr Corticosterone-Induced Lipogenesis Activation and Lipophagy Inhibition in Chicken Liver Are Alleviated by Maternal Betaine Supplementation. Authors - Yun Hu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

AHCYL1 Polyclonal antibody

AHCYL1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of HeLa using 10658-3-AP

WB analysis of Jurkat using 10658-3-AP

WB analysis of mouse thymus using 10658-3-AP

WB analysis of HeLa using 10658-3-AP

WB analysis of Jurkat using 10658-3-AP

IP experiment of HeLa using 10658-3-AP

IHC staining of human liver cancer using 10658-3-AP

IHC staining of human liver cancer using 10658-3-AP

IF Staining of HeLa using 10658-3-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Maternal Betaine Supplementation during Gestation Enhances Expression of mtDNA-Encoded Genes through D-Loop DNA Hypomethylation in the Skeletal Muscle of Newborn Piglets.

Mitotically stable modification of DNA methylation in IGF2/H19 imprinting control region is associated with activated hepatic IGF2 expression in offspring rats from betaine-supplemented dams.

Transgenerational Inheritance of Betaine-Induced Epigenetic Alterations in Estrogen-Responsive IGF-2/IGFBP2 Genes in Rat Hippocampus.

Maternal betaine supplementation in rats induces intergenerational changes in hepatic IGF-1 expression and DNA methylation.

Protective Role of IRBIT on Sodium Bicarbonate Cotransporter-n1 for Migratory Cancer Cells.

Corticosterone-Induced Lipogenesis Activation and Lipophagy Inhibition in Chicken Liver Are Alleviated by Maternal Betaine Supplementation.