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  • KD/KO Validated

AHR Polyclonal antibody

AHR Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 17840-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, CoIP, ChIP, ELISA

Ah receptor, AHR, aryl hydrocarbon receptor, bHLHe76

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, K-562 cells, MCF-7 cells
Positive IP detected inPC-3 cells
Positive IHC detected inhuman lung cancer tissue, mouse small intestine tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17840-1-AP targets AHR in WB, IHC, IF, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AHR fusion protein Ag12193 相同性解析による交差性が予測される生物種
Full Name aryl hydrocarbon receptor
Calculated molecular weight 848 aa, 96 kDa
Observed molecular weight 105-110 kDa
GenBank accession numberBC070080
Gene Symbol AHR
Gene ID (NCBI) 196
RRIDAB_2226163
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP35869
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor that has been largely regarded as a mediator of xenobiotic metabolism [PMID:18483242]. It plays a part role in physiologic activities, including attenuation of the acute phase response, cytokine signaling, T helper (TH)17 immune cell differentiation, modulation of NF-κB activity, and regulation of hormonal signaling [PMID:20423157,18540824]. It also mediates transcription factor sequestering away from a gene promoter or tethering of the AhR to a transcription factor on a promoter. AHR calculated molecular masses differ by <10%, compared with the apparent molecular masses predicted from SDS-PAGE for the two receptors (105 and 95 kDa, respectively). (PMID: 8246913)

Protocols

Product Specific Protocols
WB protocol for AHR antibody 17840-1-APDownload protocol
IHC protocol for AHR antibody 17840-1-APDownload protocol
IP protocol for AHR antibody 17840-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

J Clin Invest

Anakinra restores cellular proteostasis by coupling mitochondrial redox balance to autophagy

Authors - Frank L. van de Veerdonk
humanWB

J Immunother Cancer

GCH1 induces immunosuppression through metabolic reprogramming and IDO1 upregulation in triple-negative breast cancer.

Authors - Jin-Li Wei
  • KD Validated
human,mouseWB

Theranostics

Activating AhR alleviates cognitive deficits of Alzheimer's disease model mice by upregulating endogenous Aβ catabolic enzyme Neprilysin.

Authors - Cheng Qian
  • KD Validated
mouseWB

Br J Pharmacol

AhR-STAT3-Hmox1/Ptgs2 signaling pathway may restrict ferroptosis and improve hMSC accumulation and efficacy in mice liver

Authors - Li Han
mouseWB

mSystems

Impaired Intestinal Akkermansia muciniphila and Aryl Hydrocarbon Receptor Ligands Contribute to Nonalcoholic Fatty Liver Disease in Mice.

Authors - Zunji Shi
mouseWB,IHC,IF

J Invest Dermatol

Aryl Hydrocarbon Receptor in Cutaneous Vascular Endothelial Cells Restricts Psoriasis Development by Negatively Regulating Neutrophil Recruitment.

Authors - Zhenlai Zhu
  • KD Validated
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