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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AHR Monoclonal antibody (67785-1-Ig)

    WB analysis using 67785-1-Ig (same clone as 67785-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67785-1-Ig (AHR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67785-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using AHR Monoclonal antibody (67785-1-Ig)

    WB analysis of HSC-T6 using 67785-1-Ig (same clone as 67785-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67785-1-Ig (AHR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67785-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of 4T1 cells using AHR Monoclonal antibody (67785-1-Ig)

    WB analysis of 4T1 using 67785-1-Ig (same clone as 67785-1-PBS)

    4T1 cells were subjected to SDS PAGE followed by western blot with 67785-1-Ig (AHR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67785-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using AHR Monoclonal antibody (67785-1-Ig)

    WB analysis using 67785-1-Ig (same clone as 67785-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67785-1-Ig (AHR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67785-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using AHR Monoclonal antibody (67785-1-Ig)

    WB analysis of MCF-7 using 67785-1-Ig (same clone as 67785-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 67785-1-Ig (AHR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67785-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using AHR Monoclonal antibody (67785-1-Ig)

    WB analysis using 67785-1-Ig (same clone as 67785-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67785-1-Ig (AHR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67785-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using AHR Monoclonal antibody (67785-1-Ig)

    IHC staining of human breast cancer using 67785-1-Ig (same clone as 67785-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67785-1-Ig (AHR antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67785-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using AHR Monoclonal antibody (67785-1-Ig)

    IHC staining of human breast cancer using 67785-1-Ig (same clone as 67785-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67785-1-Ig (AHR antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67785-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using AHR Monoclonal antibody (67785-1-Ig)

    IF Staining of human breast cancer using 67785-1-Ig (same clone as 67785-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using AHR antibody (67785-1-Ig, Clone: 2D1F9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Blue (DAPI). This data was developed using the same antibody clone with 67785-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using AHR Monoclonal antibody (67785-1-Ig)

    IF Staining of mouse testis using 67785-1-Ig (same clone as 67785-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using AHR antibody (67785-1-Ig, Clone: 2D1F9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67785-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using AHR Monoclonal antibody (67785-1-Ig)

    IF Staining of HepG2 using 67785-1-Ig (same clone as 67785-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using AHR antibody (67785-1-Ig, Clone: 2D1F9 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67785-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

AHR Monoclonal antibody, PBS Only

AHR Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 67785-1-PBS
Clone No.2D1F9

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Synonyms

Class E basic helix-loop-helix protein 76, bHLHe76, Ah receptor, 2D1F9

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67785-1-PBS targets AHR in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen AHR fusion protein Ag28935 相同性解析による交差性が予測される生物種
Full Name aryl hydrocarbon receptor
Calculated molecular weight 848 aa, 96 kDa
Observed molecular weight105-110 kDa
GenBank accession numberBC070080
Gene Symbol AHR
Gene ID (NCBI) 196
RRIDAB_2918549
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP35869
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor that has been largely regarded as a mediator of xenobiotic metabolism [PMID:18483242]. It plays a part role in physiologic activities, including attenuation of the acute phase response, cytokine signaling, T helper (TH)17 immune cell differentiation, modulation of NF-κB activity, and regulation of hormonal signaling [PMID:20423157,18540824]. It also mediates transcription factor sequestering away from a gene promoter or tethering of the AhR to a transcription factor on a promoter. AHR calculated molecular masses differ by <10%, compared with the apparent molecular masses predicted from SDS-PAGE for the two receptors (105 and 95 kDa, respectively). (PMID: 8246913)