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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse testis tissue using AHR Recombinant antibody (83369-6-RR)

    IHC staining of mouse testis using 83369-6-RR (same clone as 83369-6-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83369-6-RR (AHR antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83369-6-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using AHR Recombinant antibody (83369-6-RR)

    IF Staining of HepG2 using 83369-6-RR (same clone as 83369-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using AHR antibody (83369-6-RR, Clone: 240325G9 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83369-6-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using AHR Recombinant antibody (83369-6-RR)

    IF Staining of HeLa using 83369-6-RR (same clone as 83369-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using AHR antibody (83369-6-RR, Clone: 240325G9 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83369-6-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using AHR Recombinant antibody (83369-6-RR)

    IF Staining of HeLa using 83369-6-RR (same clone as 83369-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using AHR antibody (83369-6-RR, Clone: 240325G9 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83369-6-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using AHR Recombinant antibody (83369-6-RR)

    FC experiment of HeLa using 83369-6-RR (same clone as 83369-6-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug AHR Recombinant antibody (83369-6-RR, Clone:240325G9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83369-6-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of MCF-7 cells using AHR Recombinant antibody (83369-6-RR)

    FC experiment of MCF-7 using 83369-6-RR (same clone as 83369-6-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug AHR Recombinant antibody (83369-6-RR, Clone:240325G9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83369-6-PBS in a different storage buffer formulation.

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AHR Recombinant antibody, PBS Only

AHR Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83369-6-PBS
Clone No.240325G9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, FC (Intra), ELISA

Synonyms

Class E basic helix-loop-helix protein 76, bHLHe76, Ah receptor, 240325G9

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83369-6-PBS targets AHR in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen AHR fusion protein Ag12193 相同性解析による交差性が予測される生物種
Full Name aryl hydrocarbon receptor
Calculated molecular weight 848 aa, 96 kDa
GenBank accession numberBC070080
Gene Symbol AHR
Gene ID (NCBI) 196
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP35869
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor that has been largely regarded as a mediator of xenobiotic metabolism (PMID:18483242). It plays a part role in physiologic activities, including attenuation of the acute phase response, cytokine signaling, T helper (TH) 17 immune cell differentiation, modulation of NF-κB activity, and regulation of hormonal signaling (PMID:20423157,18540824). It also mediates transcription factor sequestering away from a gene promoter or tethering of the AhR to a transcription factor on a promoter. AHR calculated molecular masses differ by <10%, compared with the apparent molecular masses predicted from SDS-PAGE for the two receptors (105 and 95 kDa, respectively). (PMID: 8246913)