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- KD/KO Validated
CoraLite® Plus 488-conjugated AHR Monoclonal antibody
AHR Monoclonal Antibody for FC (Intra)
Host / Isotype
Mouse / IgG2b
Reactivity
Human, rat
Applications
FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
2D1F9
Cat no : CL488-67785
Synonyms
Validation Data Gallery
Tested Applications
| Positive FC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-67785 targets AHR in FC (Intra) applications and shows reactivity with Human, rat samples.
| Tested Reactivity | Human, rat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | AHR fusion protein Ag28935 相同性解析による交差性が予測される生物種 |
| Full Name | aryl hydrocarbon receptor |
| Calculated molecular weight | 848 aa, 96 kDa |
| Observed molecular weight | 105-110 kDa |
| GenBank accession number | BC070080 |
| Gene symbol | AHR |
| Gene ID (NCBI) | 196 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor that has been largely regarded as a mediator of xenobiotic metabolism [PMID:18483242]. It plays a part role in physiologic activities, including attenuation of the acute phase response, cytokine signaling, T helper (TH)17 immune cell differentiation, modulation of NF-κB activity, and regulation of hormonal signaling [PMID:20423157,18540824]. It also mediates transcription factor sequestering away from a gene promoter or tethering of the AhR to a transcription factor on a promoter. AHR calculated molecular masses differ by <10%, compared with the apparent molecular masses predicted from SDS-PAGE for the two receptors (105 and 95 kDa, respectively). (PMID: 8246913)
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 AHR antibody CL488-67785 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
