Validation Data Gallery
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15642-1-AP targets AID in ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | AID fusion protein Ag8136 相同性解析による交差性が予測される生物種 |
| Full Name | activation-induced cytidine deaminase |
| Calculated molecular weight | 198 aa, 24 kDa |
| Observed molecular weight | 48-52 kDa |
| GenBank accession number | BC006296 |
| Gene Symbol | AIDA/AID |
| Gene ID (NCBI) | 57379 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9GZX7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
AID is essential to all three genetic alterations required for antigen-specific immunoglobulin: class switch recombination (CSR), somatic bypermutation (SHM), and gene conversion. Expression of AID is normally restricted to B cells in the GC, where SHM and CSR occur. It is likely these represent AID monomer and homodimer that western blot detected two bands in purified AID from nuclear extracts, one at 52 kDa and the other at 26 kDa. (PMID:16280388)