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  • Featured Product
  • KD/KO Validated

AIF Monoclonal antibody, PBS Only

AIF Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig, canine

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

8C12B2

Cat no : 67791-1-PBS

Synonyms

AIFM1, PDCD8, EC:1.6.99.-, Apoptosis-inducing factor 1, mitochondrial

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AIF Monoclonal antibody (67791-1-Ig)

    WB analysis using 67791-1-Ig (same clone as 67791-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67791-1-Ig (AIF antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67791-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using AIF Monoclonal antibody (67791-1-Ig)

    WB analysis of HeLa using 67791-1-Ig (same clone as 67791-1-PBS)

    WB result of AIF antibody (67791-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AIF transfected HeLa cells. This data was developed using the same antibody clone with 67791-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using AIF Monoclonal antibody (67791-1-Ig)

    IHC staining of human kidney using 67791-1-Ig (same clone as 67791-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67791-1-Ig (AIF antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67791-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using AIF Monoclonal antibody (67791-1-Ig)

    IHC staining of human kidney using 67791-1-Ig (same clone as 67791-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67791-1-Ig (AIF antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67791-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human kidney tissue using AIF Monoclonal antibody (67791-1-Ig)

    IF Staining of human kidney using 67791-1-Ig (same clone as 67791-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using AIF antibody (67791-1-Ig, Clone: 8C12B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67791-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human kidney tissue using AIF Monoclonal antibody (67791-1-Ig)

    IF Staining of human kidney using 67791-1-Ig (same clone as 67791-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using AIF antibody (67791-1-Ig, Clone: 8C12B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67791-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human kidney tissue using AIF Monoclonal antibody (67791-1-Ig)

    IF Staining of human kidney using 67791-1-Ig (same clone as 67791-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using AIF antibody (67791-1-Ig, Clone: 8C12B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67791-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using AIF Monoclonal antibody (67791-1-Ig)

    IF Staining of HeLa using 67791-1-Ig (same clone as 67791-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using AIF antibody (67791-1-Ig, Clone: 8C12B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67791-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using AIF Monoclonal antibody (67791-1-Ig)

    IF Staining of HeLa using 67791-1-Ig (same clone as 67791-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using AIF antibody (67791-1-Ig, Clone: 8C12B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67791-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HCT 116 cells using AIF Monoclonal antibody (67791-1-Ig)

    IF Staining of HCT 116 using 67791-1-Ig (same clone as 67791-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HCT 116 cells using AIF antibody (67791-1-Ig, Clone: 8C12B2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67791-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67791-1-PBS targets AIF in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, canine samples.

Tested Reactivity human, mouse, rat, pig, canine
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen AIF fusion protein Ag12400 相同性解析による交差性が予測される生物種
Full Name apoptosis-inducing factor, mitochondrion-associated, 1
Calculated molecular weight 609 aa, 66 kDa
Observed molecular weight 67 kDa, 57 kDa
GenBank accession numberBC111065
Gene symbol AIF
Gene ID (NCBI) 9131
RRIDAB_2918555
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Apoptosis-inducing factor (AIF) is one of the mitochondrial proteins to be released into the cytosol during apoptosis, and it is discovered as the first protein that regulates caspase-independent apoptosis(PMID:20494118). AIF is encoded as a 67 kDa protein that contains a mitochondrial localization signal (MLS) in the N-terminus.It is cleaved from the 62 kDa to the 57 kDa form following ischemic injury and translocated from the mitochondria to the nucleus in a calpain-dependent manner(PMID:19332058).