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  • Featured Product
  • KD/KO Validated

AIF Monoclonal antibody, PBS Only

AIF Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat, Pig, Canine

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

8C12B2

Cat no : 67791-1-PBS

Synonyms

AIF, AIFM1, Cell and organelle markers, PDCD8

Validation Data Gallery

Applications:
  • WB analysis using 67791-1-Ig (same clone as 67791-1-PBS)

    WB analysis using 67791-1-Ig (same clone as 67791-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67791-1-Ig (AIF antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67791-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 67791-1-Ig (same clone as 67791-1-PBS)

    WB analysis of HeLa using 67791-1-Ig (same clone as 67791-1-PBS)

    WB result of AIF antibody (67791-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AIF transfected HeLa cells. This data was developed using the same antibody clone with 67791-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 67791-1-Ig (same clone as 67791-1-PBS)

    IHC staining of human kidney using 67791-1-Ig (same clone as 67791-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67791-1-Ig (AIF antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67791-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AIFM1 antibody included
  • IHC staining of human kidney using 67791-1-Ig (same clone as 67791-1-PBS)

    IHC staining of human kidney using 67791-1-Ig (same clone as 67791-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67791-1-Ig (AIF antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67791-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AIFM1 antibody included
  • IF Staining of HeLa using 67791-1-Ig (same clone as 67791-1-PBS)

    IF Staining of HeLa using 67791-1-Ig (same clone as 67791-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using AIF antibody (67791-1-Ig, Clone: 8C12B2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67791-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 67791-1-Ig (same clone as 67791-1-PBS)

    IF Staining of HeLa using 67791-1-Ig (same clone as 67791-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using AIF antibody (67791-1-Ig, Clone: 8C12B2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67791-1-PBS in a different storage buffer formulation.

  • IF Staining of HCT 116 using 67791-1-Ig (same clone as 67791-1-PBS)

    IF Staining of HCT 116 using 67791-1-Ig (same clone as 67791-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HCT 116 cells using AIF antibody (67791-1-Ig, Clone: 8C12B2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67791-1-PBS in a different storage buffer formulation.

  • IF Staining of human kidney using 67791-1-Ig (same clone as 67791-1-PBS)

    IF Staining of human kidney using 67791-1-Ig (same clone as 67791-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using AIF antibody (67791-1-Ig, Clone: 8C12B2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67791-1-PBS in a different storage buffer formulation.

  • IF Staining of human kidney using 67791-1-Ig (same clone as 67791-1-PBS)

    IF Staining of human kidney using 67791-1-Ig (same clone as 67791-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using AIF antibody (67791-1-Ig, Clone: 8C12B2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67791-1-PBS in a different storage buffer formulation.

  • IF Staining of human kidney using 67791-1-Ig (same clone as 67791-1-PBS)

    IF Staining of human kidney using 67791-1-Ig (same clone as 67791-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using AIF antibody (67791-1-Ig, Clone: 8C12B2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67791-1-PBS in a different storage buffer formulation.

Tested Applications

Planning an IHC experiment? We recommend our IHCeasy AIFM1 Ready-To-Use IHC Kit. AIFM1 primary antibody included.

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

67791-1-PBS targets AIF in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig, Canine samples.

Tested Reactivity Human, Mouse, Rat, Pig, Canine
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen AIF fusion protein Ag12400 相同性解析による交差性が予測される生物種
Full Name apoptosis-inducing factor, mitochondrion-associated, 1
Calculated molecular weight 609 aa, 66 kDa
Observed molecular weight 67 kDa, 57 kDa
GenBank accession numberBC111065
Gene symbol AIFM1
Gene ID (NCBI) 9131
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Apoptosis-inducing factor (AIF) is one of the mitochondrial proteins to be released into the cytosol during apoptosis, and it is discovered as the first protein that regulates caspase-independent apoptosis(PMID:20494118). AIF is encoded as a 67 kDa protein that contains a mitochondrial localization signal (MLS) in the N-terminus.It is cleaved from the 62 kDa to the 57 kDa form following ischemic injury and translocated from the mitochondria to the nucleus in a calpain-dependent manner(PMID:19332058).