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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

AIFM3 Polyclonal antibody, PBS Only

AIFM3 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 14778-1-PBS

Synonyms

EC:1.-.-.-, Apoptosis-inducing factor-like protein, Apoptosis-inducing factor 3, Apoptosis inducing factor 3, AIFL

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AIFM3 Polyclonal antibody (14778-1-AP)

    WB analysis using 14778-1-AP (same clone as 14778-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14778-1-AP (AIFM3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14778-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using AIFM3 Polyclonal antibody (14778-1-AP)

    IHC staining of human ovary tumor using 14778-1-AP (same clone as 14778-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14778-1-AP (AIFM3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14778-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using AIFM3 Polyclonal antibody (14778-1-AP)

    IHC staining of human ovary tumor using 14778-1-AP (same clone as 14778-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14778-1-AP (AIFM3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14778-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using AIFM3 Polyclonal antibody (14778-1-AP)

    IHC staining of human colon cancer using 14778-1-AP (same clone as 14778-1-PBS)

    Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using 14778-1-AP (AIFM3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14778-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using AIFM3 Polyclonal antibody (14778-1-AP)

    IHC staining of human colon cancer using 14778-1-AP (same clone as 14778-1-PBS)

    Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using 14778-1-AP (AIFM3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14778-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14778-1-PBS targets AIFM3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AIFM3 fusion protein Ag6278 相同性解析による交差性が予測される生物種
Full Name apoptosis-inducing factor, mitochondrion-associated, 3
Calculated molecular weight 67 kDa
Observed molecular weight 67 kDa
GenBank accession numberBC032485
Gene symbol AIFM3
Gene ID (NCBI) 150209
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Apoptosis-inducing factor, mitochondrion-associated 3 (AIFM3) is a mitochondrial protein/flavoenzyme and its gene is located on chromosome 22q11.21. Mature human AIFM3 protein consists of 598 amino acids with a molecular weight of 66 kDa and is predominantly localized in the mitochondria. AIFM3 is widely expressed in many tissues, and breast cancer tissues and cholangiocarcinoma tissue (PMID: 32664187).