Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis using 20590-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 20590-1-AP (AIM2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis using 20590-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 20590-1-AP (AIM2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 20590-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 20590-1-AP (AIM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 20590-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 20590-1-AP (AIM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 20590-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 20590-1-AP (AIM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of SH-SY5Y using 20590-1-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20590-1-AP (AIM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of COLO 320 using 20590-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 20590-1-AP (AIM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 20590-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 20590-1-AP (AIM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 20590-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 20590-1-AP (AIM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 20590-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 20590-1-AP (AIM2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of rat testis using 20590-1-AP
rat testis tissue were subjected to SDS PAGE followed by western blot with 20590-1-AP (AIM2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human testis using 20590-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 20590-1-AP (AIM2 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human testis using 20590-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 20590-1-AP (AIM2 antibody) at dilution of 1:100 (under 40x lens).
IF Staining of MCF-7 using 20590-1-AP
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using AIM2 antibody (20590-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

Featured Product
KD/KO Validated

AIM2 Polyclonal antibody

AIM2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 20590-1-AP

Publications(30)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, ELISA

Synonyms

PYHIN4, Interferon-inducible protein AIM2

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (30)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A431 cells, HeLa cells, Jurkat cells, SH-SY5Y cells, mouse testis tissue, rat testis tissue, MCF-7 cells, A549 cells, COLO 320 cells, HepG2 cells, Raji cells
Positive IHC detected in	human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 26 publications below
IHC	See 10 publications below

Product Information

20590-1-AP targets AIM2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, zebrafish
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	AIM2 fusion protein Ag14520 相同性解析による交差性が予測される生物種
Full Name	absent in melanoma 2
Calculated molecular weight	343 aa, 39 kDa
Observed molecular weight	39-42 kDa
GenBank accession number	BC010940
Gene Symbol	AIM2
Gene ID (NCBI)	9447
RRID	AB_10694420
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O14862
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Absent in melanoma 2 (AIM2) is a sensor of cytosolic DNA that is responsible for activation of the inflammasome and host immune responses to DNA viruses and intracellular bacteria [PMID:22695634]. Upon binding to DNA, it is thought to undergo oligomerization and to associate with PYCARD initiating the recruitment of caspase-1 precusrsor and processing of interleukin-1 beta and interleukin-18. Otherwise, it functions as a tumor suppressor which may act by repressing NF-kappa-B transcriptional activity [PMID:16432157,20566831].

Protocols

Product Specific Protocols
WB protocol for AIM2 antibody 20590-1-AP	Download protocol
IHC protocol for AIM2 antibody 20590-1-AP	Download protocol
IF protocol for AIM2 antibody 20590-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Cell Mol Immunol NLRP3-dependent pyroptosis is required for HIV-1 gp120-induced neuropathology. Authors - Xiaolong He KD Validated View Article
mouse	WB	Mucosal Immunol AIM2 contributes to the maintenance of intestinal integrity via Akt and protects against Salmonella mucosal infection. Authors - G-Q Hu View Article
human	WB	J Agric Food Chem Integrative Gut Microbiota and Metabolomic Analyses Reveal the PANoptosis- and Ferroptosis-Related Mechanisms of Chrysoeriol in Inhibiting Melanoma Authors - Yuxi Liu View Article
human	IHC	Front Immunol Cytokine Secretion and Pyroptosis of Thyroid Follicular Cells Mediated by Enhanced NLRP3, NLRP1, NLRC4, and AIM2 Inflammasomes Are Associated With Autoimmune Thyroiditis. Authors - Qingling Guo View Article
human	WB	Front Cell Dev Biol Autophagy-Based Unconventional Secretory for AIM2 Inflammasome Drives DNA Damage Resistance During Intervertebral Disc Degeneration. Authors - Shuai Li KD Validated View Article
human	WB,IHC	Front Immunol Crosstalk of necroptosis and pyroptosis defines tumor microenvironment characterization and predicts prognosis in clear cell renal carcinoma Authors - Liangmin Fu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 20590-1-AP

WB analysis using 20590-1-AP

WB analysis of HepG2 using 20590-1-AP

WB analysis of HeLa using 20590-1-AP

WB analysis of Jurkat using 20590-1-AP

WB analysis of SH-SY5Y using 20590-1-AP

WB analysis of COLO 320 using 20590-1-AP

WB analysis of MCF-7 using 20590-1-AP

WB analysis of A549 using 20590-1-AP

WB analysis of mouse testis using 20590-1-AP

WB analysis of rat testis using 20590-1-AP

IHC staining of human testis using 20590-1-AP

IHC staining of human testis using 20590-1-AP

IF Staining of MCF-7 using 20590-1-AP

AIM2 Polyclonal antibody

AIM2 Polyclonal Antibody for WB, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

NLRP3-dependent pyroptosis is required for HIV-1 gp120-induced neuropathology.

AIM2 contributes to the maintenance of intestinal integrity via Akt and protects against Salmonella mucosal infection.

Integrative Gut Microbiota and Metabolomic Analyses Reveal the PANoptosis- and Ferroptosis-Related Mechanisms of Chrysoeriol in Inhibiting Melanoma

Cytokine Secretion and Pyroptosis of Thyroid Follicular Cells Mediated by Enhanced NLRP3, NLRP1, NLRC4, and AIM2 Inflammasomes Are Associated With Autoimmune Thyroiditis.

Autophagy-Based Unconventional Secretory for AIM2 Inflammasome Drives DNA Damage Resistance During Intervertebral Disc Degeneration.

Crosstalk of necroptosis and pyroptosis defines tumor microenvironment characterization and predicts prognosis in clear cell renal carcinoma