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  • Featured Product
  • KD/KO Validated

AIM2 Monoclonal antibody, PBS Only

AIM2 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, rabbit

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3E12A8

Cat no : 66902-1-PBS

Synonyms

PYHIN4, Interferon-inducible protein AIM2, 3E12A8

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AIM2 Monoclonal antibody (66902-1-Ig)

    WB analysis using 66902-1-Ig (same clone as 66902-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66902-1-Ig (AIM2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66902-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rabbit spleen tissue using AIM2 Monoclonal antibody (66902-1-Ig)

    WB analysis of rabbit spleen using 66902-1-Ig (same clone as 66902-1-PBS)

    rabbit spleen tissue were subjected to SDS PAGE followed by western blot with 66902-1-Ig (AIM2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66902-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of RAW 264.7 cells using AIM2 Monoclonal antibody (66902-1-Ig)

    WB analysis of RAW 264.7 using 66902-1-Ig (same clone as 66902-1-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66902-1-Ig (AIM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66902-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human heart tissue using AIM2 Monoclonal antibody (66902-1-Ig)

    WB analysis of human heart using 66902-1-Ig (same clone as 66902-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 66902-1-Ig (AIM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66902-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using AIM2 Monoclonal antibody (66902-1-Ig)

    IHC staining of human urothelial carcinoma using 66902-1-Ig (same clone as 66902-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 66902-1-Ig (AIM2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66902-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using AIM2 Monoclonal antibody (66902-1-Ig)

    IHC staining of human urothelial carcinoma using 66902-1-Ig (same clone as 66902-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 66902-1-Ig (AIM2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66902-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat small intestine tissue using AIM2 Monoclonal antibody (66902-1-Ig)

    IHC staining of rat small intestine using 66902-1-Ig (same clone as 66902-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 66902-1-Ig (AIM2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66902-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66902-1-PBS targets AIM2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat, rabbit samples.

Tested Reactivity human, mouse, rat, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen AIM2 fusion protein Ag14607 相同性解析による交差性が予測される生物種
Full Name absent in melanoma 2
Calculated molecular weight 343 aa, 39 kDa
Observed molecular weight 37-39 and 53 kDa
GenBank accession numberBC010940
Gene symbol AIM2
Gene ID (NCBI) 9447
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Absent in melanoma 2 (AIM2) is a sensor of cytosolic DNA that is responsible for activation of the inflammasome and host immune responses to DNA viruses and intracellular bacteria [PMID:22695634]. Upon binding to DNA, it is thought to undergo oligomerization and to associate with PYCARD initiating the recruitment of caspase-1 precusrsor and processing of interleukin-1 beta and interleukin-18. Otherwise, it functions as a tumor suppressor which may act by repressing NF-kappa-B transcriptional activity [PMID:16432157,20566831]. 66902-1-Ig antibody detects the 37-39 and 53 kDa protein in SDS-PAGE similar to the paper published. [PMID: 28809949]