AIP/ARA9 Polyclonal antibody

Name: AIP/ARA9 Polyclonal antibody
Brand: Proteintech
SKU: 18176-1-AP

AIP/ARA9 Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 18176-1-AP

Synonyms

ARA9, AIP, XAP 2, XAP2, FKBP37

Validation Data Gallery

Filter:

AllWBIHCFC (Intra)

WB analysis of HepG2 using 18176-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 18176-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 18176-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 18176-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 18176-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 18176-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 18176-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 18176-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 18176-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human liver cancer using 18176-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer using 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human liver cancer using 18176-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer using 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human heart using 18176-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human heart using 18176-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human heart using 18176-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human heart using 18176-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human heart using 18176-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human heart using 18176-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:50 (under 40x lens).
FC experiment of HeLa using 18176-1-AP
1x10^6 HeLa cells were intracellularly stained with 0.4 ug AIP/ARA9 Polyclonal antibody (18176-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Cat No. 18176-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HepG2 cells, HeLa cells, NIH/3T3 cells
Positive IHC detected in	human liver cancer tissue, human heart tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 1 publications below
IHC	See 1 publications below
IP	See 1 publications below

Product Information

18176-1-AP targets AIP/ARA9 in WB, IHC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	AIP/ARA9 fusion protein Ag12869 相同性解析による交差性が予測される生物種
Full Name	aryl hydrocarbon receptor interacting protein
Calculated molecular weight	330 aa, 38 kDa
Observed molecular weight	38 kDa
GenBank accession number	BC104797
Gene symbol	AIP
Gene ID (NCBI)	9049
RRID	AB_11182727
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for AIP/ARA9 antibody 18176-1-AP	Download protocol
IHC protocol for AIP/ARA9 antibody 18176-1-AP	Download protocol
FC protocol for AIP/ARA9 antibody 18176-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IP	J Clin Invest Anakinra restores cellular proteostasis by coupling mitochondrial redox balance to autophagy Authors - Frank L. van de Veerdonk View Article
rat	WB,IHC	Heliyon PSO/SDF-1 composite hydrogel promotes osteogenic differentiation of PDLSCs and bone regeneration in periodontitis rats Authors - Wei Zhang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

AIP/ARA9 Polyclonal antibody

AIP/ARA9 Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Validation Data Gallery

WB analysis of HepG2 using 18176-1-AP

WB analysis of HepG2 using 18176-1-AP

WB analysis of HeLa using 18176-1-AP

WB analysis of HepG2 using 18176-1-AP

WB analysis of NIH/3T3 using 18176-1-AP

WB analysis of HepG2 using 18176-1-AP

WB analysis of HeLa using 18176-1-AP

WB analysis of HepG2 using 18176-1-AP

WB analysis of NIH/3T3 using 18176-1-AP

IHC staining of human liver cancer using 18176-1-AP

IHC staining of human liver cancer using 18176-1-AP

IHC staining of human heart using 18176-1-AP

IHC staining of human heart using 18176-1-AP

IHC staining of human heart using 18176-1-AP

IHC staining of human heart using 18176-1-AP

IHC staining of human heart using 18176-1-AP

IHC staining of human heart using 18176-1-AP

FC experiment of HeLa using 18176-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Anakinra restores cellular proteostasis by coupling mitochondrial redox balance to autophagy

PSO/SDF-1 composite hydrogel promotes osteogenic differentiation of PDLSCs and bone regeneration in periodontitis rats